MSH2 Antikörper

Produktnummer ABIN4912051

Produktdetails anti-MSH2 Antikörper

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MSH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Human

Aufreinigung: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: Purified His-tagged MSH2 protein was used to produced this monoclonal antibody.

Klon: 4C2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IF()

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Andere Bezeichnung: MSH2 (MSH2 Produkte)

Synonyme: COCA1 antikoerper, FCC1 antikoerper, HNPCC antikoerper, HNPCC1 antikoerper, LCFS2 antikoerper, AI788990 antikoerper, wu:fc06b02 antikoerper, wu:fc13e09 antikoerper, zgc:55333 antikoerper, ATMSH2 antikoerper, MUTS homolog 2 antikoerper, msh2 antikoerper, mutS homolog 2 antikoerper, mutS homolog 2 (E. coli) antikoerper, MUTS homolog 2 antikoerper, mutS homolog 2 L homeolog antikoerper, MutS protein homolog 2 antikoerper, MSH2 antikoerper, Msh2 antikoerper, msh2 antikoerper, msh2.L antikoerper

Hintergrund:

Synonyms: FCC1, COCA1, HNPCC, LCFS2, HNPCC1, DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2, MSH2

Background: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Gen-ID: 4436

UniProt: P43246

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response