MSH2 Antikörper (AA 327-427)

Produktnummer ABIN6940081

Der Maus Monoklonal anti-MSH2 Antikörper (Klon MSH2-2622) (ABIN6940081) detektiert spezifisch MSH2 in WB, IHC, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Hund, Meerschweinchen und Hamster.

Kurzübersicht für MSH2 Antikörper (AA 327-427) (ABIN6940081)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reaktivität: Human, Maus, Ratte, Hund, Meerschweinchen, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MSH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: MSH2-2622

Produktdetails anti-MSH2 Antikörper

Bindungsspezifität: AA 327-427

Verwendungszweck: MSH2 (DNA Mismatch Repair Marker) Antibody

Spezifität: Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC). Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45 % of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 327-427) of human MSH2 protein (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: A549 or HepG2 cells. Colon, Thyroid, Testis or Lymph Node.

Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Andere Bezeichnung: MSH2

Hintergrund:

BAT26, COCA1, DNA mismatch repair protein Msh2, FCC1, hMSH2, HNPCC1, LCFS2, MSH2, MutS homolog 2, MutS homolog 2 colon cancer nonpolyposis type 1, MutS protein homolog 2

A549 or HepG2 cells. Colon, Testis or Lymph Node., Thyroid, Human Testis, Mouse Testis, Rat Testis, Hamster Testis, Guinea pig Testis, K-562 or HeLa

Molekulargewicht: 100kDa

Gen-ID: 4436, 597656

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response