IRF3 Antikörper

Produktnummer ABIN4904079

Produktdetails anti-IRF3 Antikörper

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IRF3 Antikörper ist unkonjugiert

Applikation: Bitte anfragen

Produktmerkmale: Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response (1-4). In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal Ser and Thr residues (between 385 and 405), leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity (5). During infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and a kinase complex that includes IKKε and TBK1 (6,7). IRF-3 is phosphorylated at Ser396 following viral infection, expression of viral nucleocapsid, and double-stranded RNA treatment. These events likely play a role in activation of IRF-3 (8).

Aufreinigung: Affinity purification

Immunogen: Recombinant protein of human IRF3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000 , IHC 1:50-1:200 , IF 1:50-1:200 , IP 1:50-1:200 Ch, IP 1:20-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C.

Details zu IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Andere Bezeichnung: IRF3 (IRF3 Produkte)

Synonyme: irf3 antikoerper, IRF3 antikoerper, C920001K05Rik antikoerper, IRF-3 antikoerper, interferon regulatory factor 3 antikoerper, interferon regulatory factor 7 antikoerper, irf3 antikoerper, IRF3 antikoerper, irf-3 antikoerper, Irf3 antikoerper, IRF7 antikoerper

Gen-ID: 3661

UniProt: Q14653

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades