PTTG1 Antikörper (Internal Region)

Produktnummer ABIN4889505

Produktdetails anti-PTTG1 Antikörper

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PTTG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Spezifität: Reacts with human 22 kDa securin

Kreuzreaktivität: Maus

Kreuzreaktivität (Details): May cross react with mouse and other species due to sequence homology.

Aufreinigung: Purified (protein A)

Immunogen: Synthetic peptide derived from internal domain of the human Securin

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- WB : 1/200 to 1/1000 - IHC: 1/50 to 1/200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Must be reconstituted in distilled water.

Buffer: Tris 0,1M, glycine 0,1M, sucrose 2 %

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Haltbarkeit: 24 months

Details zu PTTG1

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Andere Bezeichnung: Securin (PTTG1 Produkte)

Synonyme: EAP1 antikoerper, HPTTG antikoerper, PTTG antikoerper, TUTR1 antikoerper, PTTG1 antikoerper, securin antikoerper, AW555095 antikoerper, C87862 antikoerper, Pttg antikoerper, Pttg3 antikoerper, Securin antikoerper, Protein Cut2 antikoerper, pituitary tumor-transforming 1 antikoerper, pituitary tumor-transforming gene 1 antikoerper, securin, sister chromatid separation inhibitor antikoerper, PTTG1 antikoerper, Pttg1 antikoerper, cut2 antikoerper

Hintergrund: Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.

UniProt: O95997