PTTG1 Antikörper (AA 101-202)

Produktnummer ABIN735803

Produktdetails anti-PTTG1 Antikörper

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Bindungsspezifität: AA 101-202

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PTTG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Pig,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PTTG

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu PTTG1

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Andere Bezeichnung: Securin/PTTG (PTTG1 Produkte)

Synonyme: EAP1 antikoerper, HPTTG antikoerper, PTTG antikoerper, TUTR1 antikoerper, PTTG1 antikoerper, securin antikoerper, AW555095 antikoerper, C87862 antikoerper, Pttg antikoerper, Pttg3 antikoerper, Securin antikoerper, Protein Cut2 antikoerper, pituitary tumor-transforming 1 antikoerper, pituitary tumor-transforming gene 1 antikoerper, securin, sister chromatid separation inhibitor antikoerper, PTTG1 antikoerper, Pttg1 antikoerper, cut2 antikoerper

Hintergrund:

Synonyms: AW555095, C87862, Cut2, EAP 1, EAP1, ESP1 ASSOCIATED PROTEIN 1, Esp1-associated protein, hPTTG, MGC126883, MGC138276, Pds1, Pituitary tumor transforming 1, Pituitary tumor transforming protein 1, Pituitary tumor-transforming 1, isoform CRA_a, Pituitary tumor-transforming 1, isoform CRA_b, Pituitary tumor-transforming gene 1, Pituitary tumor-transforming gene 1 protein, PTTG 1, PTTG, PTTG1, PTTG1 protein, Pttg3, TUMOR TRANSFORMING 1, Tumor transforming protein 1, TUMOR-TRANSFORMING 1, TUTR 1, TUTR1.

Background: Securin is an anaphase inhibitor and plays an important role in DNA damage and spindle check point pathways. It inhibits sister chromatid separation by binding and inhibiting Esp 1, a cystein protease that causes cleavage of the cohesin Scc 1 that binds the sister chromatids together. It is responsible for targeting of Esp 1 to the nucleus and its binding to the spindle. Degradation of Securin occurs shortly before anaphase, which liberates Esp 1 and is a pre-requisite for anaphase entry. It is targeted for degradation by ubiquitination mediated bycyclosome / anaphase promoting complex (APC)functioning as a ubiquitin ligase. In response to DNA damage Chk 1 phosphorylates Securin to stabilize it against the APC mediated destruction, hence preventing the entry of such a cell into anaphase. Securin is highly expressed in various human tumors, including pituitary, adrenal, kidney, liver, and ovarian tumors, and in cell lines derived from pituitary tumors, breast tumors, endometrial tumors, and ovarian tumors.

Gen-ID: 9232