MLL4 Antikörper (C-Term)

Produktnummer ABIN488001

Produktdetails anti-MLL4 Antikörper

Target: MLL4 (Histone-Lysine N-Methyltransferase MLL4 (MLL4))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MLL4 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody reacts to MLL4.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Peptide with sequence CNCGAKRCRRFLN, from the C Terminus of the protein sequence according to NP_055542.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/128000. Western blot: Preliminary experiments gave no signal but low background in Human Heartlysates at up to 1 μg/mL. However this used our routine western blotting protocol which wewould not expect to reliably detect proteins as large as the predicted size of approx.312KDa according to NP_055542. Immunohistochemistry on paraffin sections: Human Testis shows cytoplasm staining ofspermatids. Recommended concentration: 5 - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu MLL4

Target: MLL4 (Histone-Lysine N-Methyltransferase MLL4 (MLL4))

Andere Bezeichnung: MLL4 (MLL4 Produkte)

Synonyme: HRX2 antikoerper, MLL1B antikoerper, MLL2 antikoerper, MLL4 antikoerper, TRX2 antikoerper, WBP7 antikoerper, 2610014H22Rik antikoerper, Mll2 antikoerper, Wbp7 antikoerper, mKIAA0304 antikoerper, ALR antikoerper, BC032281 antikoerper, BC058659 antikoerper, C430014K11Rik antikoerper, KMT2B antikoerper, Mll4 antikoerper, RGD1308331 antikoerper, lysine methyltransferase 2B antikoerper, lysine (K)-specific methyltransferase 2B antikoerper, lysine (K)-specific methyltransferase 2D antikoerper, KMT2B antikoerper, Kmt2b antikoerper, Kmt2d antikoerper

Hintergrund: KMT2D / MLL4 contains multiple domains including a CXXC zinc finger, three PHD zinc fingers, two FY-rich domains, and a SET (suppressor of variegation, enhancer of zeste, and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This protein is ubiquitously expressed in adult tissues where it possibly acts as a transcriptional regulatory factor. It is also expressed in solid tumor cell lines, and may be involved in human cancer. Two alternatively spliced gene transcripts encoding distinct isoforms have been reported, however, the full length nature of the shorter transcript is not known.Synonyms: HRX2, Histone-lysine N-methyltransferase MLL4, KIAA0304, KMT2D, Lysine N-methyltransferase 2D, MLL2, Myeloid/lymphoid or mixed-lineage leukemia protein 4, TRX2, Trithorax homolog 2, WBP7, WW domain-binding protein 7

Gen-ID: 9757

NCBI Accession: NP_055542

UniProt: Q9UMN6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Warburg Effekt