CD161 Antikörper (FITC)

Produktnummer ABIN487477

Produktdetails anti-CD161 Antikörper (FITC)

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD161 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Spezifität: This antibody reacts with Human CD161 antigen.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Produktmerkmale: Synonyms: HNKR-P1a, CLEC5B, NKRP1A, Killer cell lectin-like receptor subfamily B member 1, Naturalkiller cell surface protein P1A, C-type lectin domain family 5 member B

Aufreinigung: Protein-A Sepharose Chromatography.

Immunogen: Human polyclonal NK cells. Remarks: Hybridoma was established by fusion of mouse myeloma cell SP2 with Balb/cmouse splenocyte.

Klon: HP-3G10

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 10-20 μg/mL (final concentration). Positive Control: Peripheral blood Lymphocyte cells. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Flow Cytometric analysis for floating cellsProtocol 1. We use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Add 10 μL of normal goat serum to the cell pellet after tapping. Mix well, and incubatefor 5 minutes at room temperature (20~25°C). 3) Add 30 µL of the FITC labeled CD161 monoclonal antibody (10-20 μg/mL) diluted with thewashing buffer. Mix well, and incubate for 30 minutes at room temperature (20~25°C). 4) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 μL of the washing buffer and analyze by a flow cytometer. Protocol 2We usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum and 0. 1% NaN3(5x10e6 cells/mL). 3) Add 20 µL of the non diluted FITC labeled CD161 monoclonal antibody into each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Flow Cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all steps described below. 1) Add 20 μL of the non diluted FITC labeled CD161 monoclonal antibody into each tube. 2) Add 50 μL of whole blood into each tube. Mix well, and incubate for 30 minutes at roomtemperature (20~25°C). 3) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (foranalysis on BD instruments), using the procedure recommended in the respective packageinserts. 4) Resuspend the cells with 500 μL of the washing buffer [PBS containing 2% fetal calfserum (FCS) and 0. 1% NaN3] and analyze by a flow cytometer. Positive Control for Flow Cytometry: Peripheral blood Lymphocytes

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.05 mg/mL

Buffer: PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu CD161

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Andere Bezeichnung: CD161 / KLRB1 (KLRB1 Produkte)

Synonyme: NKR-P1 antikoerper, KLRB1 antikoerper, 4930431A04Rik antikoerper, Gm4696 antikoerper, Klrb1g antikoerper, Klrb6 antikoerper, Ly-55 antikoerper, Ly55 antikoerper, NKR-P1G antikoerper, Nkrp-1e antikoerper, Nkrp1g antikoerper, CD161 antikoerper, CLEC5B antikoerper, NKR antikoerper, NKR-P1A antikoerper, NKRP1A antikoerper, hNKR-P1A antikoerper, Klrb1d antikoerper, Klrb1f antikoerper, Nkr-p1e antikoerper, killer cell lectin like receptor B1 antikoerper, killer cell lectin-like receptor subfamily B member 1 antikoerper, killer cell lectin-like receptor subfamily B, member 1 antikoerper, KLRB1 antikoerper, Klrb1 antikoerper

Hintergrund: CD161, also known as NKR-P1, is a receptor originally identified on the surface of natural killer cells that is also present on some subpopulations of CD8+T cells. CD161 is often used as an NK cell marker since it is present on both immature and mature NK cells. CD161 appears to mediate NK cell activation and is up-regulated by IL-12. CD161 is expressed as a disulfide homodimer of ~60 kDa while the ligand for CD161 appears to be a carbohydrate structure that includes GalNAc and GlcNAc structures. CD161+ T cells are commonly found in human liver and intestinal epithelium where they may play a role in local immunity.Synonyms: C-type lectin domain family 5 member B, CLEC5B, HNKR-P1a, Killer cell lectin-like receptor subfamily B member 1, NKRP1A, Natural killer cell surface protein P1A

Gen-ID: 3820

UniProt: Q12918