IGFBP3 Antikörper (Biotin)

Produktnummer ABIN465183

Produktdetails anti-IGFBP3 Antikörper (Biotin)

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IGFBP3 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Spezifität: Human IGF-BP3

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IGF-BP3.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hIGF-BP3 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP3. To detect hIGF-BP3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IGF-BP3 (LS-B7473) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP3. Western Blot: To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu IGFBP3

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Andere Bezeichnung: IGFBP3 / IGFBP-3 (IGFBP3 Produkte)

Synonyme: BP-53 antikoerper, IBP3 antikoerper, AI649005 antikoerper, IGFBP-3 antikoerper, IGgfbp3 antikoerper, IGF-BP3 antikoerper, zgc:91788 antikoerper, IGFBP3 antikoerper, igfbp3 antikoerper, insulin like growth factor binding protein 3 antikoerper, insulin-like growth factor binding protein 3 antikoerper, IGFBP3 antikoerper, Igfbp3 antikoerper, igfbp3 antikoerper, IGFBP-3 antikoerper, LOC100305016 antikoerper

Hintergrund: Name/Gene ID: IGFBP3

Synonyms: IGFBP3, Binding protein 29, Binding protein 53, BP-53, IGF-binding protein 3, IGFBP-3, IBP-3, IBP3

Gen-ID: 3486

UniProt: P17936

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Growth Factor Binding