FASL Antikörper

Produktnummer ABIN465174

Produktdetails anti-FASL Antikörper

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FASL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Spezifität: Human sFas Ligand

Aufreinigung: Immunoaffinity purified

Immunogen: CHO cell derived recombinant Human sFas Ligand.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Neut (0.08 - 0.1 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.75 μg/mL-2.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a AB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of sFasL/Apo1L (10 ng/mL), a concentration of 0.08-0.1 μg/mL of this antibody is required. ELISA: To detect hsFasL/Apo1L by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sFasL/Apo1L (LS-C104462) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsFasL/Apo1L. Western Blot: To detect hsFasL/Apo1L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsFasL/Apo1L is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Andere Bezeichnung: FASLG / FASL / Fas Ligand (FASL Produkte)

Synonyme: ALPS1B antikoerper, APT1LG1 antikoerper, APTL antikoerper, CD178 antikoerper, CD95-L antikoerper, CD95L antikoerper, FASL antikoerper, TNFSF6 antikoerper, fasl antikoerper, Fas-L antikoerper, Faslg antikoerper, Tnfsf6 antikoerper, gld antikoerper, Apt1Lg1 antikoerper, Fasl antikoerper, FASLG antikoerper, zgc:162027 antikoerper, Fas ligand antikoerper, Fas ligand L homeolog antikoerper, Fas ligand (TNF superfamily, member 6) antikoerper, FASLG antikoerper, faslg.L antikoerper, Fasl antikoerper, Faslg antikoerper, faslg antikoerper

Hintergrund: Name/Gene ID: FASLG
Family: TNF

Synonyms: FASLG, ALPS1B, Apoptosis antigen ligand, APTL, CD95L, CD95 ligand, CD178 antigen, CD95-L, Fas antigen ligand, Fas ligand, FASL, TNFSF6, APT1LG1, CD178

Gen-ID: 356

UniProt: P48023

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity