DLC1 Antikörper (Isoform 1, Isoform 3, N-Term)
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- Target Alle DLC1 Antikörper anzeigen
- DLC1 (Deleted in Liver Cancer 1 (DLC1))
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Bindungsspezifität
- Isoform 1, Isoform 3, N-Term
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Reaktivität
- Human, Maus, Schwein
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser DLC1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Sequenz
- SVAIRKRSWE EHC
- Spezifität
- This antibody is expected to recognise isoforms 1 and 3 ( NP_872584.2 and NP_079043.3) but NOT isoform 2 (NP_006085.2).
- Kreuzreaktivität (Details)
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Species reactivity (expected):Mouse and Pig.
Species reactivity (tested):Human. - Aufreinigung
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- Immunogen
- Peptide with sequence from the N Terminus of the protein sequence according to NP_872584.2, NP_079043.3. Genename: DLC1
- Top Product
- Discover our top product DLC1 Primärantikörper
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- Applikationshinweise
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Peptide ELISA: Antibody Detection Limit Dilution: 1/2,000. Western Blot: Preliminary experiments gave an approx 28 kDa band in Human Liver andLung lysates as well as in different epithelial lung cell lines after 0.3 μg/mL antibodystaining. Please note that currently we cannot find an explanation in the literature for theband we observe given the calculated size of 171 kDa according to NP_872584.1 and54.8 kDa according to NP_079043.2 (isotope 3). The 28 kDa band was successfully blockedby incubation with the immunizing peptide. Immunohistochemistry: 5 μg/mL. In paraffin embedded Human Adrenal Gland (Medulla)shows strong reticulate staining in the cytoplasm of medular cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- DLC1 (Deleted in Liver Cancer 1 (DLC1))
- Andere Bezeichnung
- ARHGAP7 / DLC1 (DLC1 Produkte)
- Synonyme
- ARHGAP7 antikoerper, HP antikoerper, STARD12 antikoerper, p122-RhoGAP antikoerper, rhogap7 antikoerper, DLC-1 antikoerper, StARD12 antikoerper, Dlc-1 antikoerper, A730069N07Rik antikoerper, Arhgap7 antikoerper, dlc-1 antikoerper, RhoGAP antikoerper, Dlc1 antikoerper, DLC1 Rho GTPase activating protein antikoerper, DLC1 Rho GTPase activating protein S homeolog antikoerper, rho GTPase-activating protein 7 antikoerper, deleted in liver cancer 1 antikoerper, DLC1 antikoerper, dlc1.S antikoerper, dlc1 antikoerper, LOC100456847 antikoerper, Dlc1 antikoerper, LOC100729904 antikoerper
- Hintergrund
- DLC1 functions as a GTPase-activating protein specific for Rho and an activator of PLCD1 in vivo and induces morphological changes and detachment through cytoskeletal reorganization. DLC1 is a candidate tumor suppressor gene for human liver cancer, as well as for prostate, lung, colorectal, and breast cancers.Synonyms: Deleted in liver cancer 1 protein, HP protein, KIAA1723, Rho GTPase-activating protein 7, Rho-type GTPase-activating protein 7, STARD12, START domain-containing protein 12, StAR-related lipid transfer protein 12
- Gen-ID
- 10395
- NCBI Accession
- NP_006085
- UniProt
- Q96QB1
- Pathways
- Tube Formation, Positive Regulation of Endopeptidase Activity
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