HCN1 Antikörper (C-Term)

Produktnummer ABIN452405

Produktdetails anti-HCN1 Antikörper

Target: HCN1 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1))

Bindungsspezifität: AA 778-910, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HCN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This antibody detects HCN1 cyclic nucleotidegated Channel at ~100 kDa. No cross reactivity against HCN2.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Produktmerkmale: Synonyms: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, Braincyclic nucleotide-gated channel 1

Aufreinigung: Protein G Chromatography

Immunogen: Fusion protein amino acids 778-910 (cytoplasmic C-terminus) of rat HCN1 (also known aspotassium/sodium hyperpolarization-activated) cyclic nucleotide-gated channel

Klon: S70-28

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot: 1 - 10 μg/mL (if results are off, try using the lysate without boiling. Immunoprecipitation. Immunohistochemistry: 0.1 - 1.0 μg/mL (Perox).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS pH 7.4 containing 50 % Glycerol and 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu HCN1

Target: HCN1 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1))

Andere Bezeichnung: HCN1 / BCNG1 (HCN1 Produkte)

Synonyme: BCNG-1 antikoerper, BCNG1 antikoerper, HAC-2 antikoerper, Bcng1 antikoerper, C630013B14Rik antikoerper, HAC2 antikoerper, hyperpolarization activated cyclic nucleotide gated potassium channel 1 antikoerper, hyperpolarization-activated cyclic nucleotide-gated potassium channel 1 antikoerper, hyperpolarization-activated, cyclic nucleotide-gated K+ 1 antikoerper, hcn1 antikoerper, HCN1 antikoerper, Hcn1 antikoerper

Hintergrund: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, hyperpolarization-activated cation channels of the HCN gene family, such as HCN1, play a crucial role in the regulatons of cell excitability. Importantly, they contribute to spontaneous rhythmic activity in both the heart and brain (3).Synonyms: Brain cyclic nucleotide-gated channel 1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Gen-ID: 84390

UniProt: Q9JKB0

Pathways: Asymmetric Protein Localization