NEDD4 Antikörper (Internal Region)

Produktnummer ABIN401453

Produktdetails anti-NEDD4 Antikörper

Target: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Bindungsspezifität: Internal Region

Reaktivität: Human, Pferd, Makake, Kaninchen, Hund, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEDD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody reacts with endogenous Nedd4 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Horse, Macaque, Chimpanzee, Rabbit, Canine, Cattle.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Peptide corresponding to an internal portion of the Nedd4 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/20,000 - 1/35,000. Western Blot: 1/300 to 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.64 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu NEDD4

Target: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Andere Bezeichnung: NEDD4 (NEDD4 Produkte)

Synonyme: NEDD4-1 antikoerper, RPF1 antikoerper, AA959633 antikoerper, AL023035 antikoerper, AU019897 antikoerper, E430025J12Rik antikoerper, Nedd4-1 antikoerper, Nedd4a antikoerper, mKIAA0093 antikoerper, CG32184 antikoerper, CG42279 antikoerper, CG7555 antikoerper, DNedd4 antikoerper, Dmel\\CG42279 antikoerper, Dmel_CG32184 antikoerper, Dmel_CG7555 antikoerper, dNedd4 antikoerper, dNedd4-1 antikoerper, nedd4 antikoerper, GB16853 antikoerper, NEDD4 antikoerper, Nedd4 antikoerper, neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase antikoerper, neural precursor cell expressed, developmentally down-regulated 4 antikoerper, CG42279 gene product from transcript CG42279-RJ antikoerper, E3 ubiquitin-protein ligase Nedd-4 antikoerper, E3 ubiquitin-protein ligase NEDD4 antikoerper, NEDD4 antikoerper, Nedd4 antikoerper, LOC411723 antikoerper, LOC100457150 antikoerper, nedd4 antikoerper, LOC100547485 antikoerper

Hintergrund: NEDD4 (neural precursor expressed, developmentally down-regulated protein 4, aliases: KIAA0093, MGC176705, NEDD4-1) is a gene that is highly expressed in early embryonic central nervous system. A family of NEDD4-like proteins has more recently been defined. NEDD4 and NEDD4-like proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4-2 have been shown to down-regulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations. The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins, and ubiquitination of these proteins, mediated by the HECT domain of NEDD4, results in their internalization and removal from the plasma membrane. Mutation of the PY motifs in ENaC proteins is associated with Liddle's Syndrome, an autosomal-dominant form of hypertension. In addition to targeting sodium channels, NEDD4-2 has also been shown to negatively regulate TGF-β signaling by targeting Smad2 for degradation. Mouse and human NEDD4 are cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear.Synonyms: E3 ubiquitin-protein ligase NEDD4, KIAA0093

Gen-ID: 4734

NCBI Accession: NP_006145

UniProt: P46934

Pathways: Notch Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2