NEDD4 Antikörper (AA 801-900)

Produktnummer ABIN872620

Produktdetails anti-NEDD4 Antikörper

Target: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Bindungsspezifität: AA 801-900

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEDD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Kreuzreaktivität: Human, Ratte

Homologie: Mouse,Dog,Cow,Pig,Horse,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NEDD4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu NEDD4

Target: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Andere Bezeichnung: NEDD4 (NEDD4 Produkte)

Synonyme: NEDD4-1 antikoerper, RPF1 antikoerper, AA959633 antikoerper, AL023035 antikoerper, AU019897 antikoerper, E430025J12Rik antikoerper, Nedd4-1 antikoerper, Nedd4a antikoerper, mKIAA0093 antikoerper, CG32184 antikoerper, CG42279 antikoerper, CG7555 antikoerper, DNedd4 antikoerper, Dmel\\CG42279 antikoerper, Dmel_CG32184 antikoerper, Dmel_CG7555 antikoerper, dNedd4 antikoerper, dNedd4-1 antikoerper, nedd4 antikoerper, GB16853 antikoerper, NEDD4 antikoerper, Nedd4 antikoerper, neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase antikoerper, neural precursor cell expressed, developmentally down-regulated 4 antikoerper, CG42279 gene product from transcript CG42279-RJ antikoerper, E3 ubiquitin-protein ligase Nedd-4 antikoerper, E3 ubiquitin-protein ligase NEDD4 antikoerper, NEDD4 antikoerper, Nedd4 antikoerper, LOC411723 antikoerper, LOC100457150 antikoerper, nedd4 antikoerper, LOC100547485 antikoerper

Hintergrund:

Synonyms: RPF1, NEDD4-1, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein, Neural precursor cell expressed developmentally down-regulated protein 4, NEDD-4, NEDD4, KIAA0093, PIG53

Background: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding.

Gen-ID: 4734

UniProt: P46934

Pathways: Notch Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2