NEDD4 Antikörper (AA 173-262)

Produktnummer ABIN968557

Produktdetails anti-NEDD4 Antikörper

Target: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Bindungsspezifität: AA 173-262

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEDD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Nedd4 aa. 173-262

Klon: 15-Nedd4

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-NEDD4 Antikörper (ABIN968557)

Peruzzi, Prisco, Morrione, Valentinis, Baserga: "Anti-apoptotic signaling of the insulin-like growth factor-I receptor through mitochondrial translocation of c-Raf and Nedd4." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 25990-6, (2001) (PubMed).

Harvey, Dinudom, Komwatana, Jolliffe, Day, Parasivam, Cook, Kumar: "All three WW domains of murine Nedd4 are involved in the regulation of epithelial sodium channels by intracellular Na+." in: The Journal of biological chemistry, Vol. 274, Issue 18, pp. 12525-30, (1999) (PubMed).

Plant, Yeger, Staub, Howard, Rotin: "The C2 domain of the ubiquitin protein ligase Nedd4 mediates Ca2+-dependent plasma membrane localization." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32329-36, (1998) (PubMed).

Hatakeyama, Jensen, Weissman: "Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases." in: The Journal of biological chemistry, Vol. 272, Issue 24, pp. 15085-92, (1997) (PubMed).

Details zu NEDD4

Target: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Andere Bezeichnung: Nedd4 (NEDD4 Produkte)

Synonyme: NEDD4-1 antikoerper, RPF1 antikoerper, AA959633 antikoerper, AL023035 antikoerper, AU019897 antikoerper, E430025J12Rik antikoerper, Nedd4-1 antikoerper, Nedd4a antikoerper, mKIAA0093 antikoerper, CG32184 antikoerper, CG42279 antikoerper, CG7555 antikoerper, DNedd4 antikoerper, Dmel\\CG42279 antikoerper, Dmel_CG32184 antikoerper, Dmel_CG7555 antikoerper, dNedd4 antikoerper, dNedd4-1 antikoerper, nedd4 antikoerper, GB16853 antikoerper, NEDD4 antikoerper, Nedd4 antikoerper, neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase antikoerper, neural precursor cell expressed, developmentally down-regulated 4 antikoerper, CG42279 gene product from transcript CG42279-RJ antikoerper, E3 ubiquitin-protein ligase Nedd-4 antikoerper, E3 ubiquitin-protein ligase NEDD4 antikoerper, NEDD4 antikoerper, Nedd4 antikoerper, LOC411723 antikoerper, LOC100457150 antikoerper, nedd4 antikoerper, LOC100547485 antikoerper

Hintergrund: Regulated protein degradation involves conjugation of ubiquitin to proteins for degradation by the 26S proteasome. Protein ubiquitination is the end product of a multienzyme cascade that involves sequential thiol-ester bond interactions between ubiquitin and enzymes termed E1-E3. Neuronal precursor cell-expressed developmentally down-regulated 4 (Nedd4) is a multimodular ubiquitin protein ligase E3 that contains a Ca 2+-lipid binding domain (CaLB), 3 WW domains, and a C-terminal ubiquitin protein ligase hect domain. The CaLB is characteristic of C2 domain-containing proteins, such as PKC, phospholipase C, phospholipase A2, and synaptogamin. Nedd4 is expressed differentially in the fetal and adult brain and its highest expression is in lung and kidney. Although it localizes in the cytoplasm, increased intracellular Ca 2+ induces plasma membrane association of Nedd4. In addition, increases in intracellular Na+ leads to Nedd4 binding to epithelial Na+ channels via its WW domains, which results in targeting of Na+ channels for degradation. Thus, the subcellular localization and protein interactions of Nedd4 are regulated in a Ca2+- and Na+-dependent manner. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 110 kDa

Pathways: Notch Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2