NRF1 Antikörper (N-Term)

Produktnummer ABIN401025

Produktdetails anti-NRF1 Antikörper

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Bindungsspezifität: AA 1-534, N-Term

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Huhn, Schimpanse, Frosch

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NRF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Kreuzreaktivität (Details): Species reactivity (expected):Human, Chimpanzee, Canine, Rat, Chicken, Frog, Zebrafish.
Species reactivity (tested):Mouse.

Aufreinigung: Affinity Chromatography on Protein A.

Immunogen: Recombinant Mouse NRF1 protein corresponding to aa 1-534 of the native protein. Genename: NRF1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/3000-1/10000. Western Blot: 1/500-1/2000. Immunohistochemistry on Paraffin Sections: 2 mg/mL-5 μg/mL. Expect a band approx. 67 kDa in size corresponding to NRF1 by Western blotting in theappropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of Lower MolecularWeight bands.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 2.3 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at-20 °C. Dilute only prior to immediate use.

Details zu NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Andere Bezeichnung: NRF1 (NRF1 Produkte)

Synonyme: ALPHA-PAL antikoerper, C87038 antikoerper, D6Ertd415e antikoerper, SC:BZ3C13.1 antikoerper, SC:bZ3C13.2 antikoerper, ube2h antikoerper, Nfe2l1_retired antikoerper, IBR/F antikoerper, NRF1 antikoerper, alpha-pal antikoerper, nrf-1 antikoerper, LOC100220087 antikoerper, nuclear respiratory factor 1 antikoerper, nuclear respiratory factor 1 S homeolog antikoerper, NRF1 antikoerper, Nrf1 antikoerper, nrf1 antikoerper, nrf1.S antikoerper

Hintergrund: NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal) is the mammalian homolog to the erect wing (ewg) Drosophila protein that is required for proper development of the central nervous system and indirect flight muscles. In mammals NRF1 functions as a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. This protein links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 forms a homodimer and binds DNA as a dimer. NRF1 shows a nuclear localization and is widely expressed in embryonic, fetal, and adult tissues. Phosphorylation of NRF1 enhances DNA binding. Multiple splice variants have been identified for this protein.Synonyms: Alpha palindromic-binding protein, Alpha-pal, NRF-1, Nuclear respiratory factor 1

Gen-ID: 10090

UniProt: Q9WU00

Pathways: Regulation of Lipid Metabolism by PPARalpha