NRF1 Antikörper (Middle Region)
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- Target Alle NRF1 Antikörper anzeigen
- NRF1 (Nuclear Respiratory Factor 1 (NRF1))
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Bindungsspezifität
- AA 272-288, Middle Region
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Reaktivität
- Human, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser NRF1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Verwendungszweck
- Rabbit IgG polyclonal antibody for Nuclear respiratory factor 1(NRF1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
- Sequenz
- QHGREDLLYA FEDQQTQ
- Kreuzreaktivität (Details)
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Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - Produktmerkmale
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Rabbit IgG polyclonal antibody for Nuclear respiratory factor 1(NRF1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: nuclear respiratory factor 1
Protein Name: Nuclear respiratory factor 1 - Aufreinigung
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence in the middle region of human NRF1(272-288aa QHGREDLLYAFEDQQTQ), identical to the related rat and mouse sequences.
- Isotyp
- IgG
- Top Product
- Discover our top product NRF1 Primärantikörper
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- Applikationshinweise
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for NRF1 is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Kommentare
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Konzentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Konservierungsmittel
- Thimerosal (Merthiolate), Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Haltbarkeit
- 12 months
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Defect of mitochondrial respiratory chain is a mechanism of ROS overproduction in a rat model of alcoholic liver disease: role of zinc deficiency." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 310, Issue 3, pp. G205-14, (2016) (PubMed).
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Defect of mitochondrial respiratory chain is a mechanism of ROS overproduction in a rat model of alcoholic liver disease: role of zinc deficiency." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 310, Issue 3, pp. G205-14, (2016) (PubMed).
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- Target
- NRF1 (Nuclear Respiratory Factor 1 (NRF1))
- Andere Bezeichnung
- NRF1 (NRF1 Produkte)
- Synonyme
- ALPHA-PAL antikoerper, C87038 antikoerper, D6Ertd415e antikoerper, SC:BZ3C13.1 antikoerper, SC:bZ3C13.2 antikoerper, ube2h antikoerper, Nfe2l1_retired antikoerper, IBR/F antikoerper, NRF1 antikoerper, alpha-pal antikoerper, nrf-1 antikoerper, LOC100220087 antikoerper, nuclear respiratory factor 1 antikoerper, nuclear respiratory factor 1 S homeolog antikoerper, NRF1 antikoerper, Nrf1 antikoerper, nrf1 antikoerper, nrf1.S antikoerper
- Hintergrund
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NRF1(Nuclear Respiratory Factor 1), also known as Alpha-Pal. Gopalakrishnan and Scarpulla(1995) analyzed DNA from a panel of human/hamster cell hybrids using human-specific NRF1 PCR primers and localized the NRF1 gene to human chromosome 7. The assignment was further refined to 7q31 by cohybridization of NRF1- and chromosome 7-specific probes to human metaphase chromosomes. Efiok et al.(1994) identified genes containing alpha-Pal-binding sequences and found that these could be classified either as cellular proliferation genes, or as genes regulating the growth-responsive metabolic pathways of energy transduction, translation, and replication. Virbasius and Scarpulla(1994) noted that the nuclear-encoded mitochondrial transcription factor TFAM contains potential binding sites for NRF1, NRF2(GABPA) and SP1 within the promoter region. With use of binding and electrophoretic mobility shift assays, DNase footprinting, and mutation analysis of recombinant proteins, they demonstrated specific and functional binding of NRF1 and NRF2 to the TFAM promoter region.
Synonyms: alpha pal antibody|alpha palindromic binding protein antibody|Alpha palindromic-binding protein antibody|Alpha-pal antibody|locus control region factor 1 antibody|NFE2 related factor 1 antibody|NRF-1 antibody|Nrf1 antibody|NRF1_HUMAN antibody|nuclear factor(erythroid derived 2)-like 1 antibody|nuclear respiratory factor 1 antibody|transcription factor 11 antibody - UniProt
- Q16656
- Pathways
- Regulation of Lipid Metabolism by PPARalpha
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