ITCH Antikörper (Internal Region)

Produktnummer ABIN375211

Produktdetails anti-ITCH Antikörper

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ITCH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody reacts to ITCH.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence

Anwendungsinformationen

Applikationshinweise: ELISA: 1/32000. Western Blot: 0.03 - 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Andere Bezeichnung: ITCH / AIF4 (ITCH Produkte)

Synonyme: AIF4 antikoerper, AIP4 antikoerper, NAPP1 antikoerper, dJ468O1.1 antikoerper, 6720481N21Rik antikoerper, 8030492O04Rik antikoerper, A130065M08 antikoerper, C230047C07Rik antikoerper, itchy E3 ubiquitin protein ligase antikoerper, itchy, E3 ubiquitin protein ligase antikoerper, ITCH antikoerper, Itch antikoerper

Hintergrund: ITCH interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells (referenced from Swiss-Prot).Synonyms: Atrophin-1-interacting protein 4, E3 ubiquitin-protein ligase Itchy homolog, NAPP1, NFE2-associated polypeptide 1

Gen-ID: 83737

NCBI Accession: NP_113671

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events