IFT74 Antikörper (C-Term)

Produktnummer ABIN374425

Produktdetails anti-IFT74 Antikörper

Target: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Bindungsspezifität: C-Term

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IFT74 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: C-KTIVDALHST SGN

Spezifität: This antibody recognizes IFT74/CCDC2 at C-term.

Kreuzreaktivität (Details): Species reactivity (tested):Rat and Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_001092692.1, NP_001092693.1, NP_079379.2, AAK77221.1. Genename: IFT74

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.3-1 μg/mL. Approx 70-75 kDa band observed in lysates of cell line HEK293(calculated MW of 69.5 kDa according to AAK77221.1, but 51.5 kDa according toNP_079379.1). The observed molecular weight corresponds to earlier findings in literaturewith different antibodies (Momeni et al, BMC Neurol.2006 Dec 13,6: 44. , PMID: 17166276). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Liver shows vesicular staining in cytoplasm of hepatocytes. Immunofluorescence: Successfully used at 2.5 mg/mL to stain rat cortical neurons (see

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu IFT74

Target: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Andere Bezeichnung: IFT74 (IFT74 Produkte)

Synonyme: CCDC2 antikoerper, DKFZp469F1522 antikoerper, 1700029H06Rik antikoerper, Ccdc2 antikoerper, Cmg1 antikoerper, b2b796Clo antikoerper, CMG-1 antikoerper, CMG1 antikoerper, intraflagellar transport protein 74/72 antikoerper, intraflagellar transport 74 antikoerper, intraflagellar transport protein 74 homolog antikoerper, IFT74 antikoerper, ift74 antikoerper, LOC100632007 antikoerper, LOC100649180 antikoerper, Ift74 antikoerper

Hintergrund: Capillary morphogenesis protein 1 (CMG1) is a 600 amino acid protein and has a calculated molecular mass of 65 kD. CMG1 contains a coiled-coil domain (which shares 24 % identity with myosin heavy chain sequences from various species) and several putative phosphorylation sites. Bell et al. (2001) determined that CMG1 was 1 of several transcripts upregulated by umbilical vein endothelial cells during capillary morphogenesis in 3-dimensional collagen matrices. RT-PCR detected highest expression in adult and fetal kidney and lower expression in adult heart, placenta, lung, liver, and pancreas, and in fetal heart, lung, and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus, and spleen. Transfection into epithelial tumor cells revealed targeting of fluorescent-tagged CMG1 to an intracellular vesicular compartment.Synonyms: CCDC2, CMG-1, CMG1, Capillary morphogenesis protein 1, Coiled-coil domain-containing protein 2, Intraflagellar transport protein 74 homolog

Gen-ID: 80173

NCBI Accession: NP_001092692

UniProt: Q96LB3

Pathways: Hedgehog Signalweg, Chromatin Binding