SLC22A17 Antikörper (AA 476-489)

Produktnummer ABIN364414

Produktdetails anti-SLC22A17 Antikörper

Target: SLC22A17 (Solute Carrier Family 22 (Organic Cation Transporter), Member 17 (SLC22A17))

Bindungsspezifität: AA 476-489

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Hund, Pferd, Schwein, Affe, Fledermaus, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SLC22A17 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human SLC22A17. This antibody is expected to recognize both reported isoforms NP_065105.2 and NP_057693.3).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Pig (100%) Elephant, Rabbit (93%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-PETKRKLLPEVLRD from an internal region of human SLC22A17 (NP_065105.2, NP_057693.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Pig (100%), Elephant, Rabbit (93%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2 μg/mL), Peptide-ELISA (1:64000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2 μg/mL. Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx 150kD band in Human Cerebellum lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.7kD according to NP_065105.2. The 150kD band was successfully blocked by incubation with the immunizing peptide.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu SLC22A17

Target: SLC22A17 (Solute Carrier Family 22 (Organic Cation Transporter), Member 17 (SLC22A17))

Andere Bezeichnung: SLC22A17 (SLC22A17 Produkte)

Synonyme: 1700094C23Rik antikoerper, 24p3R antikoerper, AU041908 antikoerper, AW555662 antikoerper, BOIT antikoerper, Boct antikoerper, mBOCT antikoerper, BOCT antikoerper, NGALR antikoerper, NGALR2 antikoerper, NGALR3 antikoerper, hBOIT antikoerper, rBOCT antikoerper, solute carrier family 22 member 17 antikoerper, solute carrier family 22 (organic cation transporter), member 17 antikoerper, solute carrier family 22, member 17 antikoerper, SLC22A17 antikoerper, Slc22a17 antikoerper

Hintergrund: Name/Gene ID: SLC22A17
Subfamily: Organic cation transporter
Family: Transporter

Synonyms: SLC22A17, 24p3 receptor, 24p3R, BOIT, BOCT, HBOIT, NGALR, NGAL receptor, NGALR2, Lipocalin-2 receptor, MBOCT, NGALR3

Gen-ID: 51310

NCBI Accession: NP_065105, NP_057693

UniProt: Q8WUG5

Pathways: Transition Metal Ion Homeostasis