APOB Antikörper (AA 3984-3995)

Produktnummer ABIN364413

Produktdetails anti-APOB Antikörper

Target: APOB (Apolipoprotein B (APOB))

Bindungsspezifität: AA 3984-3995

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser APOB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human APOB.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-TDLHLRYQKDKK from an internal region of human APOB (NP_000375.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2 μg/mL. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Band corresponding to the expected size of ApoB100 is seen along with additional bands at lower molecular weights.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu APOB

Target: APOB (Apolipoprotein B (APOB))

Andere Bezeichnung: Apolipoprotein B / APOB (APOB Produkte)

Synonyme: FLDB antikoerper, LDLCQ4 antikoerper, Ac1-060 antikoerper, Apo B-100 antikoerper, ApoB-100 antikoerper, ApoB-48 antikoerper, AI315052 antikoerper, apob-100 antikoerper, apob-48 antikoerper, APOB-100 antikoerper, ApoB(100) antikoerper, APOB antikoerper, apolipoprotein B antikoerper, APOB antikoerper, Apob antikoerper

Hintergrund: Name/Gene ID: APOB

Synonyms: APOB, ApoB-100, Apolipoprotein B-48, ApoB-48, Apolipoprotein B, FLDB, LDLCQ4, Mutant Apo B 100, Apo B-100, Apolipoprotein B-100, Apolipoprotein B48

Gen-ID: 338

NCBI Accession: NP_000375

UniProt: P04114

Pathways: Lipid Metabolism