Cyclin B1 Antikörper (pSer147)
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- Target Alle Cyclin B1 (CCNB1) Antikörper anzeigen
- Cyclin B1 (CCNB1)
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Bindungsspezifität
- pSer147
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Cyclin B1 Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC), Immunofluorescence (IF)
- Spezifität
- The antibody detects endogenous level of Cyclin B1 only when phosphorylated at serine 147.
- Aufreinigung
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Immunogen
- Peptide sequence around phosphorylation site of pSer147 (A-F-S (p) -D-V) derived from Human Cyclin B1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
- Isotyp
- IgG
- Top Product
- Discover our top product CCNB1 Primärantikörper
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- Applikationshinweise
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200 - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- Target
- Cyclin B1 (CCNB1)
- Andere Bezeichnung
- Cyclin B1 (CCNB1 Produkte)
- Synonyme
- ccnb antikoerper, cycb antikoerper, cb267 antikoerper, cycb1 antikoerper, wu:fa19g04 antikoerper, wu:fb16d01 antikoerper, wu:fb16e07 antikoerper, wu:fi21c01 antikoerper, ccnb1 antikoerper, MGC53596 antikoerper, CCNB antikoerper, Ccnb1-rs1 antikoerper, Ccnb1-rs13 antikoerper, CycB1 antikoerper, Cycb-4 antikoerper, Cycb-5 antikoerper, Cycb1-rs1 antikoerper, cyclin B1 antikoerper, cyclin B1 S homeolog antikoerper, ccnb1 antikoerper, ccnb1.2 antikoerper, ccnb1.S antikoerper, CCNB1 antikoerper, Ccnb1 antikoerper, ccnb1.2.S antikoerper
- Hintergrund
- The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
- Molekulargewicht
- 60 kDa
- NCBI Accession
- NP_114172
- UniProt
- P14635
- Pathways
- Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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