DYKDDDDK Tag Antikörper

Details zu Produkt Nr. ABIN356536, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
211
116
65
20
19
11
9
8
6
3
2
1
1
1
Wirt
Kaninchen
195
150
30
30
15
Klonalität
Polyklonal
Konjugat
Unkonjugiert
40
22
19
9
9
9
9
8
8
7
7
7
7
7
7
6
5
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Western Blotting (WB)
345
126
119
102
78
53
41
14
10
7
7
6
6
4
4
3
3
3
2
2
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
Sequenz DYKDDDDK
Isotyp Ig Fraction
Reinigung Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Andere Bezeichnung DYKDDDDK Epitope Tag
Substanzklasse Tag
Hintergrund Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1).
Applikations-hinweise ELISA: 1/5,000. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.25 mg/mL
Buffer PBS containing 0.09 % (w/v) Sodium Azide as preservative.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Bilder des Herstellers
 image for anti-DYKDDDDK Tag antibody (ABIN356536) anti-DYKDDDDK Tag antibody
Allgemeine Veröffentlichungen Chubet, Brizzard: "Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells." in: BioTechniques, Vol. 20, Issue 1, pp. 136-41, 1996 (PubMed).

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