POU2AF1 Antikörper (C-Term)

Produktnummer ABIN3044424

Produktdetails anti-POU2AF1 Antikörper

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Bindungsspezifität: AA 238-256, C-Term

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POU2AF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for POU domain class 2-associating factor 1(POU2AF1) detection. Tested with WB, IHC-P in Mouse,Rat.

Sequenz: EEEESNTYEL NHTLSVEGF

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for POU domain class 2-associating factor 1(POU2AF1) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: POU class 2 associating factor 1
Protein Name: POU domain class 2-associating factor 1

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse BOB1(238-256aa EEEESNTYELNHTLSVEGF), different from the related rat sequence by one amino acid, and from human sequence by four amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu POU2AF1

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Andere Bezeichnung: POU2AF1 (POU2AF1 Produkte)

Synonyme: BOB1 antikoerper, OBF-1 antikoerper, OBF1 antikoerper, OCAB antikoerper, BOB-1 antikoerper, BOB.1 antikoerper, Bob-1 antikoerper, Bob1 antikoerper, OBF.1 antikoerper, OCA-B antikoerper, POU class 2 associating factor 1 antikoerper, POU domain, class 2, associating factor 1 antikoerper, POU2AF1 antikoerper, Pou2af1 antikoerper

Hintergrund: POU2AF1(POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al.(2002) reported that OCAB is essential for V(D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by directly enhancing Ig-kappa gene transcription in vivo. Siegel et al.(2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.

Synonyms: B cell Oct binding protein 1 antibody|B cell specific coactivator OBF 1 antibody|B cell specific coactivator OBF1 antibody|B-cell-specific coactivator OBF-1 antibody|BOB 1 antibody|BOB-1 antibody|OBF 1 antibody|OBF1 antibody|OBF1_HUMAN antibody|OCA B antibody|OCA-B antibody|OCAB antibody|OCT binding factor 1 antibody|OCT-binding factor 1 antibody|POU domain class 2 associating factor 1 antibody|POU domain class 2-associating factor 1 antibody|POU2AF1 antibody

UniProt: Q64693