GFAP Antikörper (Glial Fibrillary Acidic Protein) (AA 417-432)

Details for Product anti-GFAP Antibody No. ABIN3044350, Anbieter: Anmelden zum Anzeigen
Antigen
  • GFAP
  • AI836096
  • cb345
  • etID36982.3
  • gfapl
  • wu:fb34h11
  • wu:fk42c12
  • xx:af506734
  • zgc:110485
  • glial fibrillary acidic protein
  • GFAP
  • LOC100136168
  • gfap
  • Gfap
Epitop
AA 417-432, C-Term
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Reaktivität
Human, Maus, Ratte (Rattus)
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Wirt
Kaninchen
550
347
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Klonalität
Polyklonal
Konjugat
Dieser GFAP Antikörper ist unkonjugiert
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Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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441
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Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck Rabbit IgG polyclonal antibody for Glial fibrillary acidic protein(GFAP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human GFAP(417-432aa DGEVIKESKQEHKDVM), identical to the related rat sequence, and different from the related mouse sequence by two amino acids.
Sequenz DGEVIKESKQ EHKDVM
Isotyp IgG
Kreuzreaktivität (Details) No cross reactivity with other proteins.
Produktmerkmale Rabbit IgG polyclonal antibody for Glial fibrillary acidic protein(GFAP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: glial fibrillary acidic protein
Protein Name: Glial fibrillary acidic protein(GFAP)
Reinigung Immunogen affinity purified.
Plasmids, Primers & others Plasmide, Primers & weitere GFAP products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung GFAP (GFAP Antibody Abstract)
Hintergrund Glial fibrillary acidic protein(GFAP) is an intermediate-filament(IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. GFAP has been located in rat kidney glomeruli and peritubular fibroblasts, leydig cells of testis, skin keratinocytes, osteocytes of bones, chondrocytes of epiglottis, bronchus, and stellate-shaped cells of the pancreas and liver. Its expression is essential for normal white matter architecture and blood-brain barrier integrity, and its absence leads to late-onset CNS dysmyelination. GFAP has also been shown to play a role in mitosis by adjusting the filament network present in the cell. During mitosis, there is an increase in the amount of phosphorylated GFAP, and a movement of this modified protein to the cleavage furrow.

Synonyms: Astrocyte antibody|FLJ42474 antibody|FLJ45472 antibody|GFAP antibody|GFAP_HUMAN antibody|Glial Fibrillary Acidic Protein antibody|Intermediate filament protein antibody
UniProt P14136
Applikations-hinweise WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Kommentare

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Konzentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Konservierungs-mittel Thimerosal (Merthiolate), Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 417-432), (C-Term) antibody (ABIN3044350) Anti-GFAP antibody, IHC(P) IHC(P): Rat Brain Tissue
Western Blotting (WB) image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 417-432), (C-Term) antibody (ABIN3044350) Anti-GFAP antibody, Western blotting All lanes: Anti GFAP at 0.5ug/ml Lane 1: Rat Bra...
Western Blotting (WB) image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 417-432), (C-Term) antibody (ABIN3044350) Anti-GFAP antibody, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Brai...
Produkt verwendet in: Liu, Mei, Li, Roboti, Pang, Ying, Gao, Lowe, Bao: "Loss of the golgin GM130 causes Golgi disruption, Purkinje neuron loss, and ataxia in mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 2, pp. 346-351, 2018 (PubMed).

Xu, Hu, Jiang, Pan, Shen, Schachner: "Trimebutine, a small molecule mimetic agonist of adhesion molecule L1, contributes to functional recovery after spinal cord injury in mice." in: Disease models & mechanisms, Vol. 10, Issue 9, pp. 1117-1128, 2018 (PubMed).

Liu, Zhao, Ji, Zhu, Sui, Knight, Burnstock, He, Yuan, Xiang: "Inhibition of P2X7 receptors improves outcomes after traumatic brain injury in rats." in: Purinergic signalling, Vol. 13, Issue 4, pp. 529-544, 2018 (PubMed).

Zhou, Wang, Li, Wang, Wu, Zhang: "Paeoniflorin attenuates the neuroinflammatory response in a rat model of chronic constriction injury." in: Molecular medicine reports, Vol. 15, Issue 5, pp. 3179-3185, 2017 (PubMed).

Daugherty, Chechneva, Mayrhofer, Deng: "The hGFAP-driven conditional TSPO knockout is protective in a mouse model of multiple sclerosis." in: Scientific reports, Vol. 6, pp. 22556, 2017 (PubMed).

Mi, Gao, Liu, Ye, Li, Jin, Yang, Yang: "Cyclin-dependent kinase inhibitor flavopiridol promotes remyelination in a cuprizone induced demyelination model." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 20, pp. 2780-91, 2017 (PubMed).

Zhai, Zhao, Zhou, Lu, Zhang, Na, Feng, Qiang, Du: "The association and significance of H3K27me3 and a folate metabolic gene ACat2 in neural tube defects." in: Nutrition journal, Vol. 15, Issue 1, pp. 95, 2017 (PubMed).

Yang, Xie, Hu, Jiang, Shen, Schachner, Zhao: "CHL1 Is Expressed and Functions as a Malignancy Promoter in Glioma Cells." in: Frontiers in molecular neuroscience, Vol. 10, pp. 324, 2017 (PubMed).

Jiang, Luo, Qin, Ma, Li, Zhan, Zhang: "Electroacupuncture Suppresses the NF-κB Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats." in: Frontiers in molecular neuroscience, Vol. 10, pp. 363, 2017 (PubMed).

Tang, Luo, Qiu, Zhang, Lu, Huang, Xu, Xu: "Interactions between GHRH and GABAARs in the brains of patients with epilepsy and in animal models of epilepsy." in: Scientific reports, Vol. 7, Issue 1, pp. 18110, 2017 (PubMed).

Yuan, Hu, Gao: "Brain Localization and Neurotoxicity Evaluation of Polysorbate 80-Modified Chitosan Nanoparticles in Rats." in: PLoS ONE, Vol. 10, Issue 8, pp. e0134722, 2016 (PubMed).

Cui, Li, Gai, She, Zhang, Xu, Chen, An, Ma, Wang: "Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer's Disease-Like Amyloid-β Pathology and Neuroinflammation in the Rat Hippocampus." in: Scientific reports, Vol. 5, pp. 12943, 2016 (PubMed).

Liao, Jiang, Wang, Zhao, Chen, Li, Wang, Jie, Zhou, Zhang, Chen, Hu: "Histidine provides long-term neuroprotection after cerebral ischemia through promoting astrocyte migration." in: Scientific reports, Vol. 5, pp. 15356, 2016 (PubMed).

Zhu, Li, Luo, Wang, Li, Xie, Gao, Song, Su, Liang et al.: "Reversion of malignant phenotypes of human glioblastoma cells by β-elemene through β-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related ..." in: Journal of translational medicine, Vol. 13, pp. 356, 2016 (PubMed).

Hu, Zhang, Tian, Xun, Wang, Lv: "Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro." in: Molecular medicine reports, Vol. 13, Issue 1, pp. 49-58, 2016 (PubMed).

Xiao, Zhang, Zhang, Wang, Chen, Chen, Lv, Shi, Dong: "Re-infection of the prion from the scrapie‑infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c." in: International journal of molecular medicine, Vol. 37, Issue 3, pp. 716-26, 2016 (PubMed).

Yang, Li, Li, Feng, Liu, Han, Zhang, Qin, Zhang, Nie, Jin: "Effect of Corilagin on the Proliferation and NF-κB in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 1418309, 2016 (PubMed).

Zeng, Zhao, Ouyang, Zhao, Zhang, Chen, Yu, Lei: "Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium." in: Oncology letters, Vol. 12, Issue 2, pp. 815-824, 2016 (PubMed).

Mbori, Chuan, Feng, Alizada, Zhan: "Evaluation of the Combination of Methylprednisolone and Tranilast after Spinal Cord Injury in Rat Models." in: Journal of Korean Neurosurgical Society, Vol. 59, Issue 4, pp. 334-40, 2016 (PubMed).

Xu, Shen, Yu, Sun, Lin, Zhang: "Water-soluble ginseng oligosaccharides protect against scopolamine-induced cognitive impairment by functioning as an antineuroinflammatory agent." in: Journal of ginseng research, Vol. 40, Issue 3, pp. 211-9, 2016 (PubMed).

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