GFAP Antikörper (Glial Fibrillary Acidic Protein) (AA 417-432)

Details for Product anti-GFAP Antibody No. ABIN3044350, Anbieter: Anmelden zum Anzeigen
Antigen
  • GFAP
  • AI836096
  • cb345
  • etID36982.3
  • gfapl
  • wu:fb34h11
  • wu:fk42c12
  • xx:af506734
  • zgc:110485
  • glial fibrillary acidic protein
  • GFAP
  • LOC100136168
  • gfap
  • Gfap
Epitop
AA 417-432, C-Term
104
46
31
29
23
16
15
11
10
9
7
6
6
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
828
518
509
251
241
191
152
31
30
28
24
21
21
18
11
10
6
6
3
3
2
2
1
1
1
1
1
1
1
Wirt
Kaninchen
551
337
26
24
4
3
2
Klonalität
Polyklonal
Konjugat
Dieser GFAP Antikörper ist unkonjugiert
43
31
30
22
21
16
14
9
9
8
6
6
6
6
6
6
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
733
437
320
270
230
227
148
127
76
67
66
53
16
13
12
9
7
5
4
3
2
2
1
1
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Verwendungszweck Rabbit IgG polyclonal antibody for Glial fibrillary acidic protein(GFAP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human GFAP(417-432aa DGEVIKESKQEHKDVM), identical to the related rat sequence, and different from the related mouse sequence by two amino acids.
Sequenz DGEVIKESKQ EHKDVM
Isotyp IgG
Kreuzreaktivität (Details) No cross reactivity with other proteins.
Produktmerkmale Rabbit IgG polyclonal antibody for Glial fibrillary acidic protein(GFAP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: glial fibrillary acidic protein
Protein Name: Glial fibrillary acidic protein(GFAP)
Reinigung Immunogen affinity purified.
Plasmids, Primers & others Plasmide, Primers & weitere GFAP products on genomics-online (e.g. as negative or positive controls)
Andere Bezeichnung GFAP (GFAP Antibody Abstract)
Hintergrund Glial fibrillary acidic protein(GFAP) is an intermediate-filament(IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. GFAP has been located in rat kidney glomeruli and peritubular fibroblasts, leydig cells of testis, skin keratinocytes, osteocytes of bones, chondrocytes of epiglottis, bronchus, and stellate-shaped cells of the pancreas and liver. Its expression is essential for normal white matter architecture and blood-brain barrier integrity, and its absence leads to late-onset CNS dysmyelination. GFAP has also been shown to play a role in mitosis by adjusting the filament network present in the cell. During mitosis, there is an increase in the amount of phosphorylated GFAP, and a movement of this modified protein to the cleavage furrow.

Synonyms: Astrocyte antibody|FLJ42474 antibody|FLJ45472 antibody|GFAP antibody|GFAP_HUMAN antibody|Glial Fibrillary Acidic Protein antibody|Intermediate filament protein antibody
UniProt P14136
Applikations-hinweise WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Kommentare

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Konzentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Konservierungs-mittel Thimerosal (Merthiolate), Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 417-432), (C-Term) antibody (ABIN3044350) Anti-GFAP antibody, IHC(P) IHC(P): Rat Brain Tissue
Western Blotting (WB) image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 417-432), (C-Term) antibody (ABIN3044350) Anti-GFAP antibody, Western blotting All lanes: Anti GFAP at 0.5ug/ml Lane 1: Rat Bra...
Western Blotting (WB) image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 417-432), (C-Term) antibody (ABIN3044350) Anti-GFAP antibody, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Brai...
Produkt verwendet in: Zhou, Wang, Li, Wang, Wu, Zhang: "Paeoniflorin attenuates the neuroinflammatory response in a rat model of chronic constriction injury." in: Molecular medicine reports, Vol. 15, Issue 5, pp. 3179-3185, 2017 (PubMed).

Lu, Yuan, Ou, Cai, Wang, Sun, Zhang: "Autophagy and apoptosis during adult adipose-derived stromal cells differentiation into neuron-like cells in vitro." in: Neural regeneration research, Vol. 7, Issue 16, pp. 1205-12, 2015 (PubMed).

Liu, Zhang, Zhao, Cui, Cao, Guo: "Effects of hypothermia on S100B and glial fibrillary acidic protein in asphyxia rats after cardiopulmonary resuscitation." in: Cell biochemistry and biophysics, Vol. 71, Issue 1, pp. 401-6, 2015 (PubMed).

Liu, Cheng, Wang, Zhan, Xiong, Zhao: "Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene." in: In vitro cellular & developmental biology. Animal, Vol. 51, Issue 3, pp. 319-27, 2015 (PubMed).

Yuan, Chen, Wu, Chen, Hu, Zhang, Wang, Zhu: "The regulatory mechanism of neurogenesis by IGF-1 in adult mice." in: Molecular neurobiology, Vol. 51, Issue 2, pp. 512-22, 2015 (PubMed).

Lan, Zheng, Yang, Ji, Fan, Zeng: "Peroxisome proliferator-activated receptor-? agonist pioglitazone ameliorates white matter lesion and cognitive impairment in hypertensive rats." in: CNS neuroscience & therapeutics, Vol. 21, Issue 5, pp. 410-6, 2015 (PubMed).

Zhao, Yuan, Chen, Jiang, Liao, Wang, Zhang, Ohtsu, Hu, Chen: "Histamine promotes locomotion recovery after spinal cord hemisection via inhibiting astrocytic scar formation." in: CNS neuroscience & therapeutics, Vol. 21, Issue 5, pp. 454-62, 2015 (PubMed).

Xu, Yang, Xiong, Gu, He, Hu, Xiao, Chen, Wang: "Increased expression of receptor for activated C kinase 1 in temporal lobe epilepsy." in: Journal of neurochemistry, Vol. 133, Issue 1, pp. 134-43, 2015 (PubMed).

Zhang, Li, Zeng, Chen, Fang, Huang: "Long-term tracing of the BrdU label-retaining cells in adult rat brain." in: Neuroscience letters, Vol. 591, pp. 30-4, 2015 (PubMed).

Zhu, Dong, Shen, Bai, Zhang, Lv, Chao, Yao: "NMDA receptor NR2B subunits contribute to PTZ-kindling-induced hippocampal astrocytosis and oxidative stress." in: Brain research bulletin, Vol. 114, pp. 70-8, 2015 (PubMed).

Ge, Lei, Wang, Zhang, Han, Chen, Li, Jiang, Kang, Zhang: "miR-21 improves the neurological outcome after traumatic brain injury in rats." in: Scientific reports, Vol. 4, pp. 6718, 2014 (PubMed).

Hu, Wang, Zhang, Jiang, Zhang, Zhang, Lei, Park, Fang, Chen: "Morphology and functions of astrocytes cultured on water-repellent fractal tripalmitin surfaces." in: Biomaterials, Vol. 35, Issue 26, pp. 7386-97, 2014 (PubMed).

Sun, Cong, Liu, Jin, Si, Wang, Cai, Che, Tang, Wang, Li, Zhang: "Schisandrin A and B affect subventricular zone neurogenesis in mouse." in: European journal of pharmacology, Vol. 740, pp. 552-9, 2014 (PubMed).

Bai, Xu, Xu, Li, Qin: "Inhibition of Src phosphorylation reduces damage to the blood-brain barrier following transient focal cerebral ischemia in rats." in: International journal of molecular medicine, Vol. 34, Issue 6, pp. 1473-82, 2014 (PubMed).

Zhou, Cheng, Rao, Li, Duan: "Poly(3,4-ethylenedioxythiophene)/multiwall carbon nanotube composite coatings for improving the stability of microelectrodes in neural prostheses applications." in: Acta biomaterialia, Vol. 9, Issue 5, pp. 6439-49, 2013 (PubMed).

Wang, Li, Wang, Mei, Liu, Shen, Xiao: "Cuprizone-induced demyelination in mice: age-related vulnerability and exploratory behavior deficit." in: Neuroscience bulletin, Vol. 29, Issue 2, pp. 251-9, 2013 (PubMed).

Zhang, Xie, Wan, Ye, Guo, Lei: "Identification of U251 glioma stem cells and their heterogeneous stem-like phenotypes." in: Oncology letters, Vol. 6, Issue 6, pp. 1649-1655, 2013 (PubMed).

Liu, Zhou, Li, Liu, Huang, Zhou: "Selenoprotein S expression in the rat brain following focal cerebral ischemia." in: Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology, Vol. 34, Issue 9, pp. 1671-8, 2013 (PubMed).

Chen, Chen, Chen, Hu, Gao, Xie, Wang, Yin, Li, Wang: "ADAM17 promotes U87 glioblastoma stem cell migration and invasion." in: Brain research, Vol. 1538, pp. 151-8, 2013 (PubMed).

Chen, Pan, Georgakilas, Chen, Hu, Yang, Tian, Xia, Zhang, Cai, Ge, Yu, Zhuang: "Tetramethylpyrazine (TMP) protects cerebral neurocytes and inhibits glioma by down regulating chemokine receptor CXCR4 expression." in: Cancer letters, Vol. 336, Issue 2, pp. 281-9, 2013 (PubMed).

Haben Sie etwas anderes gesucht?