RFC1 Antikörper (N-Term)

Produktnummer ABIN3043075

Produktdetails anti-RFC1 Antikörper

Target: RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))

Bindungsspezifität: AA 46-64, N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Replication factor C subunit 1(RFC1) detection. Tested with WB, IHC-P in Human.

Sequenz: NSSRKEDDFK QKQPSKKKR

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Replication factor C subunit 1(RFC1) detection. Tested with WB, IHC-P in Human.
Gene Name: replication factor C(activator 1) 1, 145 kDa
Protein Name: Replication factor C subunit 1

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human RFC1(46-64aa NSSRKEDDFKQKQPSKKKR).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu RFC1

Target: RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))

Andere Bezeichnung: RFC1 (RFC1 Produkte)

Synonyme: A1 antikoerper, MHCBFB antikoerper, PO-GA antikoerper, RECC1 antikoerper, RFC antikoerper, RFC140 antikoerper, AtRFC1 antikoerper, replication factor C 1 antikoerper, replication factor C1 antikoerper, 140kDa antikoerper, Alp145 antikoerper, Recc1 antikoerper, Replication factor C1 antikoerper, si:dkey-170e5.1 antikoerper, zgc:162875 antikoerper, replication factor C subunit 1 antikoerper, replication factor C1 antikoerper, replication factor C (activator 1) 1 antikoerper, DNA replication factor C complex subunit Rfc1 antikoerper, RFC1 antikoerper, Rfc1 antikoerper, rfc1 antikoerper

Hintergrund: RFC(Replication Factor C), also called activator-1, is a multimeric primer-recognition protein consisting of 5 distinct subunits of 145, 40, 38, 37, and 36.5 kD. Human RFC was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40(SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.

Synonyms: A1 140 kDa subunit antibody|A1 antibody|A1 P145 Activator 1 large subunit antibody|Activator 1 140 kDa subunit antibody|Activator 1 large subunit antibody|Activator 1 subunit 1 antibody|DNA binding Protein PO GA antibody|DNA-binding protein PO-GA antibody|MHC binding factor beta antibody|MHCBFB antibody|PO GA antibody|RECC1 antibody|Replication factor C(activator 1) 1, 145 kDa antibody|Replication factor C 140 kDa subunit antibody|Replication factor C antibody|Replication factor C large subunit antibody|Replication factor C subunit 1 antibody|Replication factor C1 antibody|RF-C 140 kDa subunit antibody|RFC antibody|RFC1 antibody|RFC1_HUMAN antibody|RFC140 antibody|RFC140 Replication Factor C 140 kDa subunit antibody

UniProt: P35251

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA, Dicarboxylic Acid Transport