RFC1 Antikörper (AA 401-500)
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- Target Alle RFC1 Antikörper anzeigen
- RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))
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Bindungsspezifität
- AA 401-500
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Reaktivität
- Human, Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser RFC1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
- Kreuzreaktivität
- Human, Maus
- Homologie
- Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit
- Aufreinigung
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human RFC1
- Isotyp
- IgG
- Top Product
- Discover our top product RFC1 Primärantikörper
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- Applikationshinweise
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))
- Andere Bezeichnung
- RFC1 (RFC1 Produkte)
- Synonyme
- A1 antikoerper, MHCBFB antikoerper, PO-GA antikoerper, RECC1 antikoerper, RFC antikoerper, RFC140 antikoerper, AtRFC1 antikoerper, replication factor C 1 antikoerper, replication factor C1 antikoerper, 140kDa antikoerper, Alp145 antikoerper, Recc1 antikoerper, Replication factor C1 antikoerper, si:dkey-170e5.1 antikoerper, zgc:162875 antikoerper, replication factor C subunit 1 antikoerper, replication factor C1 antikoerper, replication factor C (activator 1) 1 antikoerper, DNA replication factor C complex subunit Rfc1 antikoerper, RFC1 antikoerper, Rfc1 antikoerper, rfc1 antikoerper
- Hintergrund
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Synonyms: DNA binding Protein PO GA, DNA-binding protein PO-GA, MHC binding factor beta, MHCBFB, RECC1, Replication factor C 140 kDa subunit, Replication factor C, Replication factor C large subunit, Replication factor C subunit 1, Replication factor C1, RF-C 140 kDa subunit, RFC1, RFC1_HUMAN, RFC140, RFC140 Replication Factor C 140 kDa subunit, A1 140 kDa subunit, A1 P145 Activator 1 large subunit, Activator 1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1.
Background: Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism, including DNA replication, DNA repair and telomere metabolism. RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction. All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide-binding consensus sequences. Four of the five RFC genes (including RFC1, RFC2, RFC3 and RFC4) have consensus ATP-binding motifs. The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not. RFC1 is a substrate for caspase-3 in vitro and is cleaved by a caspase-3-like protease during FAS-mediated apoptosis. In addition, phosphorylation of the PCNA binding domain of RFC1 by Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibits DNA synthesis. The human RFC1 gene maps to chromosome 4p14 and encodes the RFC1 subunit.
- Gen-ID
- 5981
- UniProt
- P35251
- Pathways
- Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA, Dicarboxylic Acid Transport
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