PUMA Antikörper (C-Term)

Produktnummer ABIN3042633

Produktdetails anti-PUMA Antikörper

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Bindungsspezifität: AA 145-159, C-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PUMA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Rabbit IgG polyclonal antibody for Bcl-2-binding component 3(BBC3) detection. Tested with WB in Human,Mouse,Rat.

Sequenz: ADDLNAQYER RRQEE

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Bcl-2-binding component 3(BBC3) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: BCL2 binding component 3
Protein Name: Bcl-2-binding component 3

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PUMA(145-159aa ADDLNAQYERRRQEE), identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-PUMA Antikörper (ABIN3042633)

Hui, Yuan, Ren, Jiang: "Nerve growth factor reduces apoptotic cell death in rat facial motor neurons after facial nerve injury." in: Neurosciences (Riyadh, Saudi Arabia), Vol. 20, Issue 1, pp. 65-8, (2015) (PubMed).

Zhao, Wu, Wu, Jia, Gao, Yan, Wang: "Introduction of hypoxia-targeting p53 fusion protein for the selective therapy of non-small cell lung cancer." in: Cancer biology & therapy, Vol. 11, Issue 1, pp. 95-107, (2011) (PubMed).

Details zu PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Andere Bezeichnung: BBC3 (BBC3 Produkte)

Synonyme: BBC3 antikoerper, Puma antikoerper, JFY-1 antikoerper, JFY1 antikoerper, PUMA antikoerper, PUMA/JFY1 antikoerper, BCL2 binding component 3 antikoerper, Bcl-2 binding component 3 antikoerper, BBC3 antikoerper, Bbc3 antikoerper, bbc3 antikoerper

Hintergrund: The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91 % amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.

Synonyms: BBC 3 antibody|BBC3 antibody|BBC3_HUMAN antibody|BCL 2 binding component 3 antibody|Bcl-2-binding component 3 antibody|BCL2 binding component 3 antibody|JFY 1 antibody|JFY-1 antibody|JFY1 antibody|p53 up regulated modulator of apoptosis antibody|p53 up-regulated modulator of apoptosis antibody|p53 Upregulated Modulator of Apoptosis antibody|PUMA alpha antibody|PUMA/JFY1 antibody

Pathways: p53 Signalweg, Positive Regulation of Endopeptidase Activity