Immunofluorescence. Immunohistochemistry on paraffin sections: 5 μg/mL. ELISA: 1: 5000 - 1: 40000. Western Blot: 1: 500 - 1: 3000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Upon receipt store the antibody (in aliquots) at-20 °C. Dilute only prior to immediate use.
Target
RAP1 (TERF2IP)
(Telomeric Repeat Binding Factor 2, Interacting Protein (TERF2IP))
Rif1 (also known as telomere-associated protein RIF1 and Rap1-interacting factor 1) is a mouse ortholog of the yeast Rif1 family of telomere-associated proteins identified on the basis of its high expression in primordial germ cells and embryo-derived pluripotent stem cell lines. Mouse Rif1 is a protein involved in negative regulation of telomere length. It is also localized to ATM dependent DNA damage foci and may act at the chromatin level to allow access to some factors.Synonyms: PP8000, TRF2-interacting telomeric protein Rap1, Telomeric repeat-binding factor 2-interacting protein 1