CD35 Antikörper
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- Target Alle CD35 (CR1) Antikörper anzeigen
- CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))
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Reaktivität
- Human, Pavian, Cynomolgus, Rhesusaffen
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD35 Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Produktmerkmale
- This antibody recognizes a protein of 210-220 kDa, which is identified as the complement receptor 1 (CR1), or CD35. The antibody is specific for a site on CD35 that is distal from the C3b-binding site, so that it is unable to block CD35 activity. The CD35 antibody is highly specific and shows no cross-reaction with CR2. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.
- Aufreinigung
- Protein G affinity chromatography
- Immunogen
- Intact human monocytes were used as the immunogen for this CD35 antibody.
- Klon
- E11
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product CR1 Primärantikörper
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- Applikationshinweise
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store the CD35 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))
- Andere Bezeichnung
- CD35 (CR1 Produkte)
- Synonyme
- C3BR antikoerper, C4BR antikoerper, CD35 antikoerper, KN antikoerper, C3DR antikoerper, CD21 antikoerper, Cr-1 antikoerper, Cr-2 antikoerper, Cr1 antikoerper, CR1-L antikoerper, CR2 antikoerper, CR1 antikoerper, complement C3b/C4b receptor 1 (Knops blood group) antikoerper, complement receptor 2 antikoerper, complement component (3d/Epstein Barr virus) receptor 2 antikoerper, complement receptor type 1 antikoerper, CR1 antikoerper, Cr2 antikoerper, CR2 antikoerper, LOC718120 antikoerper
- Hintergrund
- This antibody recognizes a protein of 210-220 kDa, which is identified as the complement receptor 1 (CR1), or CD35. The antibody is specific for a site on CD35 that is distal from the C3b-binding site, so that it is unable to block CD35 activity. The CD35 antibody is highly specific and shows no cross-reaction with CR2. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.
- Gen-ID
- 1378
- Pathways
- Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity
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