GFAP Antikörper
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- Target Alle GFAP Antikörper anzeigen
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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Reaktivität
- Human, Ratte, Maus, Schwein, Rind (Kuh), Huhn, Kaninchen
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser GFAP Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Produktmerkmale
- This antibody recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein. The mAb shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. It is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. GFAP antibody is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
- Aufreinigung
- Protein G affinity chromatography
- Immunogen
- GFAP isolated from pig spinal cord was used as the immunogen.
- Klon
- GA-5
- Isotyp
- IgG1
- Top Product
- Discover our top product GFAP Primärantikörper
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- Applikationshinweise
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the GFAP antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store the GFAP antibody at 2-8°C.
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- Target
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
- Andere Bezeichnung
- GFAP (GFAP Produkte)
- Synonyme
- GFAP antikoerper, AI836096 antikoerper, cb345 antikoerper, etID36982.3 antikoerper, gfapl antikoerper, wu:fb34h11 antikoerper, wu:fk42c12 antikoerper, xx:af506734 antikoerper, zgc:110485 antikoerper, glial fibrillary acidic protein antikoerper, GFAP antikoerper, LOC100136168 antikoerper, gfap antikoerper, Gfap antikoerper
- Hintergrund
- This antibody recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein. The mAb shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. It is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. GFAP antibody is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
- Gen-ID
- 2670
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