PODXL Antikörper (Extracellular Domain, Internal Region)

Produktnummer ABIN3025407

Produktdetails anti-PODXL Antikörper

Target: PODXL (Podocalyxin-Like (PODXL))

Bindungsspezifität: Extracellular Domain, Internal Region

Reaktivität: Human, Ratte, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PODXL Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: PEG precipitation

Immunogen: A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human PODXL was used as the immunogen for the Podocalyxin antibody.

Klon: 2A4

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Podocalyxin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Podocalyxin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Andere Bezeichnung: Podocalyxin (PODXL Produkte)

Synonyme: Gp200 antikoerper, PC antikoerper, PCLP antikoerper, PCLP-1 antikoerper, PCB antikoerper, AW121214 antikoerper, Ly102 antikoerper, Pclp1 antikoerper, podocalyxin antikoerper, MEP21 antikoerper, PODXL antikoerper, Gp135 antikoerper, PCLP1 antikoerper, cPCLP1 antikoerper, podxl antikoerper, fc18d02 antikoerper, wu:fc18d02 antikoerper, Pc antikoerper, Pcb antikoerper, Podxl antikoerper, podocalyxin like antikoerper, pyruvate carboxylase antikoerper, podocalyxin-like antikoerper, PODXL antikoerper, PC antikoerper, Podxl antikoerper, podxl antikoerper, Pcx antikoerper

Hintergrund: Podocalyxin/PODXL is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Pathways: Tube Formation