NAPSA Antikörper

Produktnummer ABIN3025326

Produktdetails anti-NAPSA Antikörper

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NAPSA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G affinity chromatography

Immunogen: A recombinant fragment from the human protein was used as the immunogen for the Napsin A antibody.

Klon: NAPSA-1238

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Napsin A antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Napsin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu NAPSA

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Andere Bezeichnung: Napsin A (NAPSA Produkte)

Synonyme: KAP antikoerper, Kdap antikoerper, NAP1 antikoerper, NAPA antikoerper, SNAPA antikoerper, pronapsin antikoerper, kap antikoerper, kdap antikoerper, nap1 antikoerper, napa antikoerper, napsa antikoerper, snapa antikoerper, napsin A aspartic peptidase antikoerper, napsin A aspartic peptidase L homeolog antikoerper, NAPSA antikoerper, Napsa antikoerper, napsa.L antikoerper

Hintergrund: Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Pathways: Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis