Smooth Muscle Actin Antikörper (N-Term)

Produktnummer ABIN3024126

Produktdetails anti-Smooth Muscle Actin Antikörper

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Bindungsspezifität: N-Term

Reaktivität: Human, Ratte, Maus, Huhn, Rind (Kuh), Kaninchen, Affe, Pavian, Meerschweinchen, Hund, Cat, Schwein, Ziege, Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Smooth Muscle Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G affinity chromatography

Immunogen: An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth muscle actin antibody.

Klon: 1A4

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Variations in protocols, secondaries and substrates may require the Smooth muscle actin antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for the immunohistochemical staining of FFPE tissue.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC(FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the Smooth muscle actin antibody at 2-8°C (aliquot/freeze the azide-free format).

Details zu Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Andere Bezeichnung: Smooth Muscle Actin (ACTA2 Produkte)

Synonyme: AAT6 antikoerper, ACTSA antikoerper, MYMY5 antikoerper, GAMMA-ACTIN antikoerper, ACT antikoerper, ACTA3 antikoerper, ACTE antikoerper, ACTL3 antikoerper, ACTSG antikoerper, Act-4 antikoerper, Act4 antikoerper, SMGA antikoerper, 0610041G09Rik antikoerper, Actvs antikoerper, SMalphaA antikoerper, a-SMA antikoerper, alphaSMA antikoerper, wu:fb63d03 antikoerper, ASMA antikoerper, SMactin antikoerper, a-sma antikoerper, aat6 antikoerper, actsa antikoerper, mymy5 antikoerper, sm-actin antikoerper, ACTA2 antikoerper, actc1 antikoerper, ACT-4 antikoerper, asma antikoerper, ACTGE antikoerper, actin, alpha 2, smooth muscle, aorta antikoerper, actin, gamma 2, smooth muscle, enteric antikoerper, actin, alpha 2, smooth muscle, aorta L homeolog antikoerper, ACTA2 antikoerper, ACTG2 antikoerper, Actg2 antikoerper, Acta2 antikoerper, acta2 antikoerper, acta2.L antikoerper

Hintergrund: Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin.

Gen-ID: 59

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development