Smooth Muscle Actin Antikörper (N-Term)

Produktnummer ABIN6940521

Produktdetails anti-Smooth Muscle Actin Antikörper

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Bindungsspezifität: N-Term

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Smooth Muscle Actin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Spezifität: Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Kreuzreaktivität (Details): Predicted to show a broad reactivity.

Aufreinigung: Purified by Protein A/G

Immunogen: N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH (1A4), Recombinant full-length human ACTA2 protein (ACTA2/791)

Klon: 1A4-ACTA2-791

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), ,Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Andere Bezeichnung: ACTA2 (ACTA2 Produkte)

Synonyme: AAT6 antikoerper, ACTSA antikoerper, MYMY5 antikoerper, GAMMA-ACTIN antikoerper, ACT antikoerper, ACTA3 antikoerper, ACTE antikoerper, ACTL3 antikoerper, ACTSG antikoerper, Act-4 antikoerper, Act4 antikoerper, SMGA antikoerper, 0610041G09Rik antikoerper, Actvs antikoerper, SMalphaA antikoerper, a-SMA antikoerper, alphaSMA antikoerper, wu:fb63d03 antikoerper, ASMA antikoerper, SMactin antikoerper, a-sma antikoerper, aat6 antikoerper, actsa antikoerper, mymy5 antikoerper, sm-actin antikoerper, ACTA2 antikoerper, actc1 antikoerper, ACT-4 antikoerper, asma antikoerper, ACTGE antikoerper, actin, alpha 2, smooth muscle, aorta antikoerper, actin, gamma 2, smooth muscle, enteric antikoerper, actin, alpha 2, smooth muscle, aorta L homeolog antikoerper, ACTA2 antikoerper, ACTG2 antikoerper, Actg2 antikoerper, Acta2 antikoerper, acta2 antikoerper, acta2.L antikoerper

Molekulargewicht: 42kDa

Gen-ID: 59

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development