DKK1 Antikörper (AA 250-262)

Produktnummer ABIN296913

Produktdetails anti-DKK1 Antikörper

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Bindungsspezifität: AA 250-262

Reaktivität: Human, Maus, Hund, Pferd, Schwein, Rind (Kuh), Affe, Ziege

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DKK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human DKK1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Bovine, Dog, Goat, Elephant, Panda, Horse, Pig (100%) Gibbon, Rat, Bat, Hamster, Platypus (92%) Rabbit (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-DHHQASNSSRLHT from the C-terminus of human DKK1 (NP_036374.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Bovine, Dog, Goat, Elephant, Panda, Horse, Pig (100%), Gibbon, Rat, Bat, Hamster, Platypus (92%), Rabbit (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), Peptide-ELISA (1:128000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Andere Bezeichnung: DKK1 (DKK1 Produkte)

Synonyme: DKK-1 antikoerper, SK antikoerper, AI414495 antikoerper, SGY antikoerper, SGY-1 antikoerper, SGY1 antikoerper, Soggy antikoerper, mSgy antikoerper, dkk1 antikoerper, dkk antikoerper, dkk-1 antikoerper, xdkk1 antikoerper, Xdkk-1 antikoerper, Dickkopf1 antikoerper, dickkopf-1 antikoerper, mdkk-1 antikoerper, dkk1-A antikoerper, dickkopf WNT signaling pathway inhibitor 1 antikoerper, dickkopf like acrosomal protein 1 antikoerper, dickkopf-related protein 1 antikoerper, dickkopf-like 1 antikoerper, dickkopf WNT signaling pathway inhibitor 1b antikoerper, dickkopf protein antikoerper, dickkopf WNT signaling pathway inhibitor 1 S homeolog antikoerper, DKK1 antikoerper, Dkk1 antikoerper, DKKL1 antikoerper, LOC100228789 antikoerper, Dkkl1 antikoerper, dkk1b antikoerper, dkk1 antikoerper, dickkopf antikoerper, dkk1.S antikoerper

Hintergrund: Name/Gene ID: DKK1

Synonyms: DKK1, Dickkopf-1 like, DKK-1, Dickkopf-1, Dickkopf-related protein 1, Dickkopf related protein-1, HDkk-1, Hdkk1, Dickkopf-like protein 1, SK

Gen-ID: 22943

NCBI Accession: NP_036374

UniProt: O94907

Pathways: WNT Signalweg, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation