LIG4 Antikörper

Produktnummer ABIN2857063

Produktdetails anti-LIG4 Antikörper

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIG4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Human, Maus

Produktmerkmale: Rabbit polyclonal antibody to DNA ligase IV (ligase IV, DNA, ATP-dependent)
DNA ligase IV antibody [N2C2], Internal

Aufreinigung: Purified by antigen-affinity chromatography.

Güteklasse: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human DNA ligase IV. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Molt-4

Validation: KO/KD

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.65 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-LIG4 Antikörper (ABIN2857063)

Ly, Teitz, Kim, Shoshani, Skaletsky, Fachinetti, Page, Cleveland: "Selective Y centromere inactivation triggers chromosome shattering in micronuclei and repair by non-homologous end joining." in: Nature cell biology, Vol. 19, Issue 1, pp. 68-75, (2017) (PubMed).

Zhu, Fisher, Bessho, Peng: "Protein phosphatase 1 and phosphatase 1 nuclear targeting subunit-dependent regulation of DNA-dependent protein kinase and non-homologous end joining." in: Nucleic acids research, Vol. 45, Issue 18, pp. 10583-10594, (2017) (PubMed).

Details zu LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Andere Bezeichnung: DNA ligase 4 (LIG4 Produkte)

Synonyme: zgc:165595 antikoerper, lig4 antikoerper, DNA LIGASE IV antikoerper, LIG4 antikoerper, lig4-A antikoerper, 5830471N16Rik antikoerper, tiny antikoerper, DNL4 antikoerper, ligase IV, DNA, ATP-dependent antikoerper, DNA ligase 4 antikoerper, ATP-dependent DNA ligase implicated in dsDNA break repair via nonhomologous end-joining antikoerper, DNA ligase IV antikoerper, ligase IV, DNA, ATP-dependent L homeolog antikoerper, lig4 antikoerper, LIG4 antikoerper, LOC100212302 antikoerper, CpipJ_CPIJ005161 antikoerper, PTRG_09982 antikoerper, MCYG_05151 antikoerper, LOC657210 antikoerper, ATLIG4 antikoerper, lig4.L antikoerper, Lig4 antikoerper

Hintergrund: The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

Cellular Localization: Nucleus

Molekulargewicht: 104 kDa

Gen-ID: 3981

UniProt: P49917

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response