ATP6V1H Antikörper (Center)

Produktnummer ABIN2856776

Produktdetails anti-ATP6V1H Antikörper

Target: ATP6V1H (ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H))

Bindungsspezifität: Center

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATP6V1H Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Kreuzreaktivität: Huhn, Schwein, Xenopus laevis, Zebrafisch (Danio rerio), Rind (Kuh), Xenopus tropicalis

Kreuzreaktivität (Details): Chicken (96 %), Pig (99 %), Xenopus laevis (94 %), Zebrafish (91 %), Bovine (98 %), Xenopus tropicalis (94 %)

Produktmerkmale: Rabbit Polyclonal antibody to ATP6V1H (ATPase, H+ transporting, lysosomal 50/57 kDa, V1 subunit H)
ATP6V1H antibody [N3C3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ATP6V1H. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IFAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: 293T , A431 , H1299 , HeLa , Raji , mouse brain

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.56 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu ATP6V1H

Target: ATP6V1H (ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H))

Andere Bezeichnung: ATP6V1H (ATP6V1H Produkte)

Synonyme: MSTP042 antikoerper, NBP1 antikoerper, SFD antikoerper, SFDalpha antikoerper, SFDbeta antikoerper, VMA13 antikoerper, V-ATPASE antikoerper, 0710001F19Rik antikoerper, AU022349 antikoerper, CGI-11 antikoerper, chunp6890 antikoerper, ATPase H+ transporting V1 subunit H antikoerper, ATPase, H+ transporting, lysosomal V1 subunit H antikoerper, ATP6V1H antikoerper, Atp6v1h antikoerper, atp6v1h antikoerper

Hintergrund: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase. Three alternatively spliced transcript variants encode two isoforms of the H subunit.

Molekulargewicht: 56 kDa

Gen-ID: 51606

Pathways: Transition Metal Ion Homeostasis, Proton Transport