DFFB Antikörper (Middle Region)

Produktnummer ABIN2784747

Produktdetails anti-DFFB Antikörper

Target: DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))

Bindungsspezifität: Middle Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DFFB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: EYFYGLLFTS ENLKLVHIVC HKKTTHKLNC DPSRIYKPQT RLKRKQPVRK

Homologie: Human: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against DFFB. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DFFB

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 338 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu DFFB

Target: DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))

Andere Bezeichnung: DFFB (DFFB Produkte)

Synonyme: CAD antikoerper, CPAN antikoerper, DFF-40 antikoerper, DFF2 antikoerper, DFF40 antikoerper, LOC100223514 antikoerper, 40kDa antikoerper, 5730477D02Rik antikoerper, Didff antikoerper, Cad antikoerper, carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase antikoerper, DNA fragmentation factor subunit beta antikoerper, DNA fragmentation factor, beta polypeptide (caspase-activated DNase) antikoerper, DNA fragmentation factor, beta subunit antikoerper, CAD antikoerper, DFFB antikoerper, dffb antikoerper, LOC100223514 antikoerper, Dffb antikoerper

Hintergrund: DFFB is a nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. DFFB degrades naked DNA and induces apoptotic morphology.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CAD, CPAN, DFF-40, DFF2, DFF40
Protein Interaction Partner: DFFA, UBC, NUBP1, MARCKS, APP, CAND1, COPS5, CUL1, CUL2, CUL5, NEDD8, CIDEB, DFFB, TOP2A,
Protein Size: 338

Molekulargewicht: 39 kDa

Gen-ID: 1677

NCBI Accession: NM_004402, NP_004393

UniProt: O76075

Pathways: Apoptose, Caspase Kaskade in der Apoptose