Granulocytes Antikörper
Reaktivität: Ratte
FACS, IP
Wirt: Maus
Monoclonal
RP
unconjugated
(1 reference)-
- Target
- Granulocytes
-
Reaktivität
- Ratte
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Unkonjugiert
-
Applikation
- Flow Cytometry (FACS), Immunoprecipitation (IP)
- Marke
- BD Pharmingen™
- Produktmerkmale
-
The RP-1 antibody reacts with rat peritoneal and peripheral blood neutrophils and bone marrow cells. It does not bind rat macrophages, rat eosinophils, or peritoneal neutrophils from mice, rabbits, guinea pigs, or human peripheral blood as determined by antibody adsorption experiments. Flow cytometric analysis of peripheral blood leukocytes indicates that all granulocytes (cells having high side scatter), but not lymphocytes or monocytes (low side-scatter cells), express that antigen recognized by RP-1 mAb. Expression of the RP-1 antigen on rat peritoneal neutrophils is enhanced by stimulation with PMA or ConA. Immunoprecipitation of rat PMA-activated and non-treated neutrophil membranes with the RP-1 mAb produced two bands of approximately 85 kDa in m.w. However, the RP-1 antigen has not been biochemically characterized.
BD Pharmingen™ Purified Mouse Anti-Rat Granulocytes - Purified - Clone RP-1 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.5 mg - Aufreinigung
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- WKA/Hok rat peritoneal neutrophils
- Klon
- RP
- Isotyp
- IgG2a kappa
-
anti-Granulocytes antibody
Reaktivität: Human, Makake FACS, IHC (p), IF Wirt: Maus Monoclonal SPM250 unconjugated
anti-Granulocytes antibody (Biotin)Reaktivität: Ratte FACS, IHC (p), IHC (fro) Wirt: Maus Monoclonal RK-4 Biotin
-
- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Konzentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store undiluted at 4°C.
-
-
: "Selective depletion of rat neutrophils by in vivo administration of a monoclonal antibody." in: Journal of leukocyte biology, Vol. 46, Issue 2, pp. 96-102, (1989) (PubMed).
-
: "Selective depletion of rat neutrophils by in vivo administration of a monoclonal antibody." in: Journal of leukocyte biology, Vol. 46, Issue 2, pp. 96-102, (1989) (PubMed).
-
- Target
- Granulocytes
-
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