Granulocytes Antikörper (Biotin)

Produktnummer ABIN111864

Produktdetails

Target: Granulocytes

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Aufreinigung: Affinity Chromatography

Immunogen: Peritoneal cells.

Klon: RK-4

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1/50-1/100. Immunohistochemistry on frozen sections: 0.75 μg/mL (1/400),does not react on routinely processed paraffin sections. Suggested positive control: Rat spleen, blood smears. Antigen Distribution on Isolated Cells: Only a low percentage of cells from peripheralblood stains positive. Positive on peritoneal cells 18h after thioglycollate injection (95 % granulocytes). Negative on peritoneal cells 4 days after thioglycollate injection. Negative on isolated lymphocytes, monocytes,erythrocytes and platelets. See Table 1. for details.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.3 mg/mL IgG

Buffer: Stock Solution contains PBS, pH 7.2 with 0.01 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Antigendetails

Target: Granulocytes

Hintergrund: Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules, Eosinophils have prominent granules that stain readily with the acid dye eosin, and Basophils have prominent granules that stain readily basic (non acidic) dyes.Synonyms: Granulocyte cells