LKB1 Antikörper (pSer428)

Produktnummer ABIN2568294

Produktdetails anti-LKB1 Antikörper

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Bindungsspezifität: pSer428

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LKB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: The antibody detects endogenous level of LKB1 only when phosphorylated at serine 428.

Aufreinigung: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: LKB1 (phospho Ser428) antibody was raised against a peptide sequence around phosphorylation site of serine428 (R-L-S (p)-A-C) derived from Human LKB1 .

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1:500~1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store antibody at -20°C for up to one year.

Details zu LKB1

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Andere Bezeichnung: LKB1 (STK11 Produkte)

Synonyme: pjs antikoerper, LKB1 antikoerper, XEEK1 antikoerper, Stk11 antikoerper, PJS antikoerper, hLKB1 antikoerper, AA408040 antikoerper, Lkb1 antikoerper, Par-4 antikoerper, R75140 antikoerper, mLKB1 antikoerper, wu:fj61a05 antikoerper, zgc:110180 antikoerper, xeek1 antikoerper, serine/threonine kinase 11 antikoerper, polarization-related protein LKB1 antikoerper, serine/threonine kinase 11 L homeolog antikoerper, STK11 antikoerper, stk11 antikoerper, LOC662493 antikoerper, Stk11 antikoerper, stk11.L antikoerper

Hintergrund: Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, leading to promote their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2: it thereby regulates inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neurons polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53, the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1 Also acts as a mediator p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways.

Molekulargewicht: 65 kDa

Gen-ID: 6794

NCBI Accession: NP_000446

UniProt: Q15831

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt