Dieser Serotonin Receptor 3A Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Spezifität
This antibody is expected to recognize both reported isoforms (NP_998786.1 and NP_000860.1).
Aufreinigung
Serotonin receptor 3A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Serotonin receptor 3A antibody was raised against a 12 amino acid synthetic peptide near the internal region of Serotonin receptor 3A.
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 55 kDa band observed in human colon lysates (calculated MW of 55.3 kDa according to NP_000860.1. Recommended concentration: 0.3-1 μg/mL. A minor band of unknown identity was also consistently observed at 200 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.