Nitrotyrosine Antikörper (Biotin)

Produktnummer ABIN2192140

Produktdetails anti-Nitrotyrosine Antikörper (Biotin)

Target: Nitrotyrosine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nitrotyrosine Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)

Sterilität: 0.2 μm filtered

Klon: HM-11

Anwendungsinformationen

Applikationshinweise: For immuno histology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Warning: keep sterile, do not add sodium azide or any nitric containing preservative since this will affect the antibody!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.1 % bovine serum albumin.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-Nitrotyrosine Antikörper (Biotin) (ABIN2192140)

ter Steege, Koster-Kamphuis, van Straaten, Forget, Buurman: "Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA." in: Free radical biology & medicine, Vol. 25, Issue 8, pp. 953-63, (1999) (PubMed).

Kooy, Royall, Ye, Kelly, Beckman: "Evidence for in vivo peroxynitrite production in human acute lung injury." in: American journal of respiratory and critical care medicine, Vol. 151, Issue 4, pp. 1250-4, (1995) (PubMed).

Beckmann, Ye, Anderson, Chen, Accavitti, Tarpey, White: "Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry." in: Biological chemistry Hoppe-Seyler, Vol. 375, Issue 2, pp. 81-8, (1994) (PubMed).

Beckman, Beckman, Chen, Marshall, Freeman: "Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 4, pp. 1620-4, (1990) (PubMed).

Details zu Nitrotyrosine

Target: Nitrotyrosine

Abstract: Nitrotyrosine Produkte

Substanzklasse: Chemical

Hintergrund: This highly specific monoclonal antibody HM.11 reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.