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Nitrotyrosine Antikörper (Biotin) Primary Antibody

IA, IHC (fro), IHC (p), WB Wirt: Maus Monoclonal HM-11 Biotin
Pubmed (4 Referenzen)
Produktnummer ABIN2192140
$466.27
Zzgl. Versandkosten $45.00
50 μg
Lieferung in 6 bis 8 Werktagen
  • Target Alle Nitrotyrosine Antikörper anzeigen
    Nitrotyrosine
    Reaktivität
    Bitte anfragen
    Wirt
    • 32
    • 26
    • 8
    Maus
    Klonalität
    • 34
    • 31
    Monoklonal
    Konjugat
    • 33
    • 7
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Nitrotyrosine Antikörper ist konjugiert mit Biotin
    Applikation
    • 50
    • 33
    • 26
    • 23
    • 22
    • 16
    • 13
    • 13
    • 13
    • 11
    • 10
    • 8
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Sterilität
    0.2 μm filtered
    Klon
    HM-11
  • Applikationshinweise
    For immuno histology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Warning: keep sterile, do not add sodium azide or any nitric containing preservative since this will affect the antibody!
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    PBS, containing 0.1 % bovine serum albumin.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
    Haltbarkeit
    12 months
  • ter Steege, Koster-Kamphuis, van Straaten, Forget, Buurman: "Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA." in: Free radical biology & medicine, Vol. 25, Issue 8, pp. 953-63, (1999) (PubMed).

    Kooy, Royall, Ye, Kelly, Beckman: "Evidence for in vivo peroxynitrite production in human acute lung injury." in: American journal of respiratory and critical care medicine, Vol. 151, Issue 4, pp. 1250-4, (1995) (PubMed).

    Beckmann, Ye, Anderson, Chen, Accavitti, Tarpey, White: "Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry." in: Biological chemistry Hoppe-Seyler, Vol. 375, Issue 2, pp. 81-8, (1994) (PubMed).

    Beckman, Beckman, Chen, Marshall, Freeman: "Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 4, pp. 1620-4, (1990) (PubMed).

  • Target
    Nitrotyrosine
    Abstract
    Nitrotyrosine Produkte
    Substanzklasse
    Chemical
    Hintergrund
    This highly specific monoclonal antibody HM.11 reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.
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