For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. It is recommended that solutions with a calcium concentration of 1 mM are used (2B4 is a calcium- dependent antibody).
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Haltbarkeit
12 months
Liu, Jensen, Hansen, Petersen, Takahashi, Ezekowitz, Hansen, Jensenius, Thiel: "Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses." in: Scandinavian journal of immunology, Vol. 53, Issue 5, pp. 489-97, (2001) (PubMed).
Sastry, Zahedi, Lelias, Whitehead, Ezekowitz: "Molecular characterization of the mouse mannose-binding proteins. The mannose-binding protein A but not C is an acute phase reactant." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 2, pp. 692-7, (1991) (PubMed).
Target
Mbl1
(Mannose-Binding Lectin (Protein A) 1 (Mbl1))
Mannose Binding Lectin (MBL) also called mannose- or mannan-binding protein (MBP) is a member of the group of collectins. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N- acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. It forms a complex with C1r/C1s like serine proteases designated MASPs that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q-independent. MBL exhibits complement-dependent antibacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The mouse forms are known as MBL-A and MBL-C. The MBL-C concentrations in serum are about 6-fold compared to that of MBL-A. MBL-A, but not MBL-C was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A. The monoclonal antibody 2B4 is a calcium-dependent antibody.