beta Actin Antikörper (AA 359-368)

Produktnummer ABIN214319

Produktdetails anti-beta Actin Antikörper

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Bindungsspezifität: AA 359-368

Reaktivität: Human, Maus, Ratte, Affe, Schwein, Schaf, Rind (Kuh), Hamster, Meerschweinchen, Ziege, Frosch

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser beta Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Amino acids 359-368 of Human beta Actin.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from human ACTB / Beta-actin.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:10000 - 1:40000), IF (1:500 - 1:2000), IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:4000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.Antibody has been tested in IHC with human and frog samples and in Western blot with human and mouse samples. It is expected to react with a wide range of species due to sequence homology.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: ACTB / Beta Actin (ACTB Produkte)

Synonyme: BRWS1 antikoerper, PS1TP5BP1 antikoerper, ACTA1 antikoerper, Actx antikoerper, E430023M04Rik antikoerper, beta-actin antikoerper, ps1tp5bp1 antikoerper, act-2 antikoerper, actb antikoerper, B-actin antikoerper, LOC100174883 antikoerper, LOC100304816 antikoerper, A antikoerper, ACT5C antikoerper, Ac5C antikoerper, Act antikoerper, Act-5C antikoerper, Act5 antikoerper, Act5c antikoerper, Actin antikoerper, Actin/BAP47 antikoerper, Actin5C antikoerper, BAP47 antikoerper, Bap47 antikoerper, CG4027 antikoerper, Dmel\\CG4027 antikoerper, M32055 antikoerper, T11 antikoerper, act antikoerper, act 5C antikoerper, act5C antikoerper, actin antikoerper, actin5C antikoerper, anon-EST:fe2D2 antikoerper, beta-actin/Bap47 antikoerper, cyt5C antikoerper, l(1)G0009 antikoerper, l(1)G0010 antikoerper, l(1)G0025 antikoerper, l(1)G0079 antikoerper, l(1)G0117 antikoerper, l(1)G0177 antikoerper, l(1)G0245 antikoerper, l(1)G0330 antikoerper, l(1)G0420 antikoerper, l(1)G0486 antikoerper, Bact antikoerper, ACT-5 antikoerper, ACTB antikoerper, B-ACTZF antikoerper, actba antikoerper, bact antikoerper, bactin1 antikoerper, bactzf antikoerper, wu:fd18f05 antikoerper, A26C1B antikoerper, POTE2alpha antikoerper, POTEACTIN antikoerper, Beta-actin antikoerper, actin beta antikoerper, actin, beta antikoerper, cytoplasmic actin OlCA1 antikoerper, beta-actin antikoerper, Actin 5C antikoerper, actin gamma 1 antikoerper, actin, beta 1 antikoerper, actin, beta L homeolog antikoerper, beta actin antikoerper, POTE ankyrin domain family member F antikoerper, actin, beta like 2 antikoerper, actin, cytoplasmic 1 antikoerper, ACTB antikoerper, Actb antikoerper, actb antikoerper, LOC100174883 antikoerper, LOC100304816 antikoerper, Act5C antikoerper, ACTG1 antikoerper, actb1 antikoerper, actb.L antikoerper, LOC100136352 antikoerper, POTEF antikoerper, ACTBL2 antikoerper, LOC109073280 antikoerper

Hintergrund: Name/Gene ID: ACTB

Synonyms: ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1

Gen-ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction