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beta Actin Antikörper (N-Term)

Name: beta Actin Antikörper (N-Term)
Brand: antibodies-online
SKU: ABIN2854709
Price: 630.63 EUR
Availability: InStock

KO Validated ACTB Reaktivität: Human WB, IF, IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated

WB Image Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE Beta-actin antibody antibody diluted at 1:1000

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin, antibody (Green) at 1:1000 dilution.

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Actin, antibody at 1:500 dilution.

IHC

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE antibody diluted at 1:5000

WB Image Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody , diluted at 1:1000.

IHC-P Image beta Actin antibody detects beta Actin protein at cytosol on mouse prostate by immunohistochemical analysis.

IHC

6 Abbildungen

(4 Referenzen)

Produktnummer ABIN2854709

Datenblatt (PDF)

Target Alle beta Actin (ACTB) Antikörper anzeigen

beta Actin (ACTB) (Actin, beta (ACTB))
Bindungsspezifität
39

18

16

6

6

5

5

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reaktivität
190

135

121

64

51

44

37

36

32

25

20

20

12

10

9

8

7

6

5

5

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1
Human
Wirt
113

93

5

2

1
Kaninchen
Passende Sekundärantikörper
Klonalität
107

105
Polyklonal
Konjugat
133

15

14

10

4

4

4

4

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Dieser beta Actin Antikörper ist unkonjugiert
Applikation
177

76

58

49

36

32

29

24

13

13

7

6

6

5

4

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Kreuzreaktivität

Cat, Hund, Drosophila melanogaster, Human, Maus

Produktmerkmale

Rabbit Polyclonal antibody to beta Actin (actin, beta)
beta Actin antibody

Aufreinigung

Purified by antigen-affinity chromatography.

Güteklasse

KO Validated

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human beta Actin. The exact sequence is proprietary.

Isotyp

IgG

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anti-Actin, beta (ACTB) antibody
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anti-Actin, beta (ACTB) (AA 359-368) antibody
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anti-Actin, beta (ACTB) (AA 2-16) antibody
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anti-Actin, beta (ACTB) antibody
ACTB Reaktivität: Human, Maus, Ratte WB, IF, IHC (p), FACS, DNA Mic Wirt: Maus Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh) WB, IHC (p) Wirt: Maus Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reaktivität: Human WB, ELISA, IHC (p) Wirt: Maus Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reaktivität: Human, Maus, Huhn, Kaninchen, Schwein, Rind (Kuh), Hamster, Ziege, Fisch WB, ELISA Wirt: Maus Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB Reaktivität: Human WB, ELISA, IHC, IF, IP, FACS Wirt: Maus Monoclonal 1D12E12B5 unconjugated

Applikationshinweise

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare

Positive Control: NIH-3T3 , Raji , 293T
Validation: KO/KD

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1.16 mg/mL

Buffer

1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Yu, Luan, Liu, Su, Li: "MiR-296 Promotes Osteoblast Differentiation by Upregulating Cbfal." in: Pharmacology, Vol. 105, Issue 3-4, pp. 190-201, (2020) (PubMed).

Tao, Cheng, Yang: "Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway." in: Clinical interventions in aging, Vol. 15, pp. 373-385, (2020) (PubMed).

Cai, Zhang, Zhang, Chen, Hu: "Effect of Th9/IL-9 on the growth of gastric cancer in nude mice." in: OncoTargets and therapy, Vol. 12, pp. 2225-2234, (2019) (PubMed).

Li, Yu, Jiao, Wang, Zhang, Sun: "Vandetanib (ZD6474) induces antiangiogenesis through mTOR-HIF-1 alpha-VEGF signaling axis in breast cancer cells." in: OncoTargets and therapy, Vol. 11, pp. 8543-8553, (2018) (PubMed).
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung

actin beta (ACTB Produkte)

Synonyme

BRWS1 antikoerper, PS1TP5BP1 antikoerper, ACTA1 antikoerper, Actx antikoerper, E430023M04Rik antikoerper, beta-actin antikoerper, ps1tp5bp1 antikoerper, act-2 antikoerper, actb antikoerper, B-actin antikoerper, LOC100174883 antikoerper, LOC100304816 antikoerper, A antikoerper, ACT5C antikoerper, Ac5C antikoerper, Act antikoerper, Act-5C antikoerper, Act5 antikoerper, Act5c antikoerper, Actin antikoerper, Actin/BAP47 antikoerper, Actin5C antikoerper, BAP47 antikoerper, Bap47 antikoerper, CG4027 antikoerper, Dmel\\CG4027 antikoerper, M32055 antikoerper, T11 antikoerper, act antikoerper, act 5C antikoerper, act5C antikoerper, actin antikoerper, actin5C antikoerper, anon-EST:fe2D2 antikoerper, beta-actin/Bap47 antikoerper, cyt5C antikoerper, l(1)G0009 antikoerper, l(1)G0010 antikoerper, l(1)G0025 antikoerper, l(1)G0079 antikoerper, l(1)G0117 antikoerper, l(1)G0177 antikoerper, l(1)G0245 antikoerper, l(1)G0330 antikoerper, l(1)G0420 antikoerper, l(1)G0486 antikoerper, Bact antikoerper, ACT-5 antikoerper, ACTB antikoerper, B-ACTZF antikoerper, actba antikoerper, bact antikoerper, bactin1 antikoerper, bactzf antikoerper, wu:fd18f05 antikoerper, A26C1B antikoerper, POTE2alpha antikoerper, POTEACTIN antikoerper, Beta-actin antikoerper, actin beta antikoerper, actin, beta antikoerper, cytoplasmic actin OlCA1 antikoerper, beta-actin antikoerper, Actin 5C antikoerper, actin gamma 1 antikoerper, actin, beta 1 antikoerper, actin, beta L homeolog antikoerper, beta actin antikoerper, POTE ankyrin domain family member F antikoerper, actin, beta like 2 antikoerper, actin, cytoplasmic 1 antikoerper, ACTB antikoerper, Actb antikoerper, actb antikoerper, LOC100174883 antikoerper, LOC100304816 antikoerper, Act5C antikoerper, ACTG1 antikoerper, actb1 antikoerper, actb.L antikoerper, LOC100136352 antikoerper, POTEF antikoerper, ACTBL2 antikoerper, LOC109073280 antikoerper

Hintergrund

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Cellular Localization: Cytoplasm , cytoskeleton

Molekulargewicht

42 kDa

Gen-ID

60

UniProt

P60709

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction