Glucocorticoid Receptor Antikörper

Produktnummer ABIN211167

Produktdetails anti-Glucocorticoid Receptor Antikörper

Target: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Reaktivität: Maus, Ratte, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Glucocorticoid Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Spezifität: Recognizes glucocorticoid receptor, Mr 85-100kD. Species cross-reactivity: Rat, mouse and hamster. Does not react with human.

Aufreinigung: Protein G purified

Immunogen: Native glucocorticoid receptor purified from rat liver.

Type of Immunogen: Purified protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: IP, WB

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot Analysis: 1-8 μg/mL of this lot detected Glucocorticoid Receptor in RIPA lysates from L6, PC12, and 3T3/A31 cells. L6 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Glucocorticoid Receptor (4 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates glucocorticoid receptor (~97kD). Immunoprecipitation: This antibody has been reported by an independent laboratory to immunoprecipitate glucocorticoid receptor from WEHI-7 and WCL2 cells.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Glucocorticoid Receptor

Target: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Andere Bezeichnung: NR3C1/Glucocorticoid Receptor (NR3C1 Produkte)

Synonyme: GR antikoerper, gcr antikoerper, grl antikoerper, xGR antikoerper, gccr antikoerper, gr antikoerper, zgc:113038 antikoerper, NR3C1 antikoerper, GCL antikoerper, LOC100302444 antikoerper, Gcr antikoerper, Grl antikoerper, Grl-1 antikoerper, Grl1 antikoerper, GRL antikoerper, GCCR antikoerper, GCR antikoerper, NO antikoerper, GR-A antikoerper, nr3c1 antikoerper, nuclear receptor subfamily 3 group C member 1 antikoerper, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antikoerper, nuclear receptor subfamily 3, group C, member 1 antikoerper, glucocorticoid receptor antikoerper, NR3C1 antikoerper, nr3c1 antikoerper, LOC100302444 antikoerper, Nr3c1 antikoerper

Hintergrund: Name/Gene ID: NR3C1
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: NR3C1, Glucocorticoid Receptor beta, GR, GCCR, GCR, Glucocorticoid receptor, Glucocorticoid receptor alpha, GRL

Gen-ID: 2908

UniProt: P04150

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process