Glucocorticoid Receptor Antikörper (AA 1-373)

Produktnummer ABIN3043453

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Glucocorticoid Receptor in WB, IHC, IF, ICC und FACS. Es zeigt Reaktivität gegenüber Proben von Human.Es wurde in 1 Publikation zitiert.

Kurzübersicht für Glucocorticoid Receptor Antikörper (AA 1-373) (ABIN3043453)

Target: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glucocorticoid Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-Glucocorticoid Receptor Antikörper

Bindungsspezifität: AA 1-373

Verwendungszweck: Anti-Glucocorticoid Receptor/NR3C1 Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Glucocorticoid Receptor/NR3C1 Antibody (ABIN3043453). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NR3C1 recombinant protein (Position: M1-D373). Human NR3C1 shares 85% and 82% amino acid (aa) sequences identity with mouse and rat NR3C1, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Glucocorticoid Receptor Antikörper (ABIN3043453)

Sharma, Maras, Das, Hussain, Mishra, Shasthry, Sharma, Weiss, Elkrief, Rautou, Gilgenkrantz, Lotersztajn, Paradis, de la Grange, Junot, Moreau, Sarin: "Pre-therapy liver transcriptome landscape in Indian and French patients with severe alcoholic hepatitis and steroid responsiveness." in: Scientific reports, Vol. 7, Issue 1, pp. 6816, (2017) (PubMed).

Details zu Glucocorticoid Receptor

Target: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Andere Bezeichnung: NR3C1

Hintergrund:

Synonyms: Glucocorticoid receptor,GR,Nuclear receptor subfamily 3 group C member 1,NR3C1,GRL,

Tissue Specificity: Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. .

Background: The glucocorticoid receptor (GR, or GCR), also known as NR3C1, is the receptor to which cortisol and other glucocorticoids bind. In humans, the GR protein is encoded by NR3C1 gene which is located on chromosome 5 (5q31). GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response. Because the receptor gene is expressed in several forms, it has many different (pleiotropic) effects in different parts of the body. The activated GR complex up-regulates the expression of anti-inflammatory proteins in the nucleus or represses the expression of pro-inflammatory proteins in the cytosol (by preventing the translocation of other transcription factors from the cytosol into the nucleus).

Sequence Similarities: Belongs to the nuclear hormone receptor family. NR3 subfamily.

Molekulargewicht: 95 kDa

Gen-ID: 2908

UniProt: P04150

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process