CD45 Antikörper

Produktnummer ABIN1981850

Produktdetails anti-CD45 Antikörper

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reaktivität: Human, Maus, Cat

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD45 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Functional Studies (Func)

Spezifität: The rat monoclonal antibody RA3-6B2 recognizes an extracellular epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.

Kreuzreaktivität (Details): Human, Mouse, Feline (Cat)

Aufreinigung: Purified by protein-G affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Endotoxin-Niveau: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Abelson murine leukemia virus-induced pre-B tumor cells

Klon: RA3-6B2

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Functional application: Modulation of B cell responses.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Konservierungsmittel: Azide free

Handhabung: Do not freeze. Do not use after expiration date stamped on vial label.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD45 Antikörper (ABIN1981850)

Lochhead, Sonderegger, Ma, Brewster, Cornwall, Maylor-Hagen, Miller, Zachary, Weis, Weis: "Endothelial cells and fibroblasts amplify the arthritogenic type I IFN response in murine Lyme disease and are major sources of chemokines in Borrelia burgdorferi-infected joint tissue." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 5, pp. 2488-501, (2012) (PubMed).

Montecino-Rodriguez, Leathers, Dorshkind: "Identification of a B-1 B cell-specified progenitor." in: Nature immunology, Vol. 7, Issue 3, pp. 293-301, (2006) (PubMed).

Rothaeusler, Baumgarth: "Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 69, Issue 4, pp. 249-59, (2006) (PubMed).

Cappione, Pugh-Bernard, Anolik, Sanz: "Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 7, pp. 4298-307, (2004) (PubMed).

Tsurui, Nishimura, Hattori, Hirose, Okumura, Shirai: "Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 48, Issue 5, pp. 653-62, (2000) (PubMed).

Rada, Ehrenstein, Neuberger, Milstein: "Hot spot focusing of somatic hypermutation in MSH2-deficient mice suggests two stages of mutational targeting." in: Immunity, Vol. 9, Issue 1, pp. 135-41, (1998) (PubMed).

Watanabe, Akaike: "Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells." in: Scandinavian journal of immunology, Vol. 39, Issue 5, pp. 419-25, (1994) (PubMed).

Details zu CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Andere Bezeichnung: CD45R (PTPRC Produkte)

Synonyme: PTPRC antikoerper, si:dkeyp-47c5.1 antikoerper, B220 antikoerper, CD45 antikoerper, CD45R antikoerper, GP180 antikoerper, L-CA antikoerper, LCA antikoerper, LY5 antikoerper, T200 antikoerper, Cd45 antikoerper, Ly-5 antikoerper, Lyt-4 antikoerper, loc antikoerper, Lca antikoerper, RT7 antikoerper, cd45 antikoerper, protein tyrosine phosphatase, receptor type C antikoerper, protein tyrosine phosphatase, receptor type, C antikoerper, receptor-type tyrosine-protein phosphatase C antikoerper, PTPRC antikoerper, ptprc antikoerper, LOC100074271 antikoerper, Ptprc antikoerper

Hintergrund: CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.,PTPRCR, T200R

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling