PUMA Antikörper (AA 76-170)

Produktnummer ABIN1870134

Produktdetails anti-PUMA Antikörper

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Bindungsspezifität: AA 76-170

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PUMA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Verwendungszweck: Polyclonal Antibody to p53 Upregulated Modulator Of Apoptosis (PUMA)

Spezifität: The antibody is a rabbit polyclonal antibody raised against PUMA. It has been selected for its ability to recognize PUMA in immunohistochemical staining and western blotting.

Kreuzreaktivität: Human, Maus

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant p53 Upregulated Modulator Of Apoptosis (PUMA) corresdonding to Arg76~Val170

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western blotting: 0.5-2 μg/mL

1:280-1100 Immunohistochemistry: 5-20 μg/mL

1:28-110 Immunocytochemistry: 5-20 μg/mL

1:28-110 Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Andere Bezeichnung: p53 Upregulated Modulator Of Apoptosis (BBC3 Produkte)

Synonyme: BBC3 antikoerper, Puma antikoerper, JFY-1 antikoerper, JFY1 antikoerper, PUMA antikoerper, PUMA/JFY1 antikoerper, BCL2 binding component 3 antikoerper, Bcl-2 binding component 3 antikoerper, BBC3 antikoerper, Bbc3 antikoerper, bbc3 antikoerper

Hintergrund: JFY1, BBC3, BCL2 Binding Component 3

Pathways: p53 Signalweg, Positive Regulation of Endopeptidase Activity